pepkio-elisa-plate-mapper

Programmatically construct, validate, and quality-check 96-well and 384-well microplate layouts for ELISA assays and bioanalytical workflows via the Pepkio API.

What It Does

pepkio-elisa-plate-mapper provides a Python interface to programmatically design and validate microplate layouts for enzyme-linked immunosorbent assays (ELISA), spectrophotometric protein quantification, and serial dilution series. It checks well classifications, verifies essential blank controls, evaluates perimeter edge-effect vulnerabilities, and computes intra-assay coefficient of variation (%CV) across replicate groups.

Features

• Typed Pydantic Models: Programmatically define plate layouts using ElisaPlateMapperInput and WellInput models.
• Assay Quality Control Checks: Automatically detect missing blank controls and verify standard curve point counts.
• Perimeter Edge-Effect Assessment: Compute perimeter standard fraction to identify standards positioned in outer wells subject to evaporation bias.
• Replicate Intra-Assay Precision: Track %CV across replicate groups to evaluate pipetting consistency.
• CLI & REST API Client: Access manifest examples, fetch past runs, and validate plate configurations synchronously or asynchronously.

Installation

pip install pepkio-elisa-plate-mapper

Quick Example

from pepkio_elisa_plate_mapper import ElisaPlateMapperInput, PepkioClient, WellInput

# Define wells for a microplate layout
wells = [
    WellInput(well="A1", type="standard", label="Std 100 ng/mL", concentration=100.0, od=1.85, group_id="std_1", replicate=1),
    WellInput(well="A2", type="standard", label="Std 100 ng/mL", concentration=100.0, od=1.82, group_id="std_1", replicate=2),
    WellInput(well="H12", type="blank", label="Blank", od=0.04),
    WellInput(well="B1", type="unknown", label="Serum Sample A", od=0.92, group_id="samp_A", replicate=1),
]

input_data = ElisaPlateMapperInput(wells=wells)

# Execute layout validation via the Pepkio API client
with PepkioClient(api_key="YOUR_API_KEY") as client:
    result = client.run(input_data)
    print("Run Status:", result.status)
    print("QC Results:", result.result)

Typical Use Cases

• Sandwich & Indirect ELISA: Organize standard curves, antibody dilutions, and unknown serum/plasma samples in duplicate or triplicate.
• Colorimetric Protein Assays: Map BSA standard series and cell lysate replicates for BCA, Bradford, or Lowry spectrophotometric assays.
• Serial Dilutions & Standard Curves: Plan 8-point or 12-point serial dilution series while avoiding outer row/column thermal gradients.
• qPCR & RT-qPCR Plate Layouts: Arrange standard template dilutions and No Template Control (NTC) wells across 96-well or 384-well plates.
• Cell Viability Screens: Organize drug candidate serial dilutions and vehicle controls for MTT or Luminescent cell-based assays.

Scientific Background

Microplate assays rely on consistent spatial arrangement and accurate control wells for reliable quantification. Thermal gradients, differential evaporation along perimeter wells (rows A/H and columns 1/12), and inconsistent pipetting in technical replicates are major sources of intra-assay variance. Structured microplate mapping ensures systematic control placement, early detection of missing blanks, and quantitative monitoring of perimeter exposure and intra-assay precision (%CV) prior to downstream curve fitting.

Web Application

For researchers who prefer a graphical interface, an interactive web version is available.

Web Application: https://www.pepkio.com/tools/elisa-plate-mapper

Web-only features include an interactive drag-and-drop 96-well grid visualization, automatic layout generation, printable bench worksheet protocols, and shareable setup links.

Documentation and Resources

GitHub Repository: https://github.com/pepkio/pepkio-elisa-plate-mapper

Web Application: https://www.pepkio.com/tools/elisa-plate-mapper

Source code and issue tracking are maintained on GitHub.

About Pepkio

Pepkio (https://www.pepkio.com/) develops software tools and provides bioinformatics analysis services (https://www.pepkio.com/cro) for life science research. Explore additional online laboratory calculators and microplate utility tools at https://www.pepkio.com.

Keywords

• ELISA
• microplate
• plate mapper
• 96-well plate
• 384-well plate
• standard curve
• serial dilution
• well assignment
• intra-assay CV
• edge effect
• optical density
• absorbance
• bioanalysis
• qPCR layout
• BCA assay
• Bradford assay
• antibody dilution
• microplate reader
• quality control
• pipetting layout
• assay validation
• technical replicates
• blank controls
• laboratory calculator
• Python client
• ELISA plate layout generator
• 96 well microplate layout mapper
• standard curve serial dilution planner
• microplate edge effect detector
• intra assay coefficient of variation calculator
• microplate reader sample well validator
• sandwich ELISA layout design tool
• qPCR plate setup generator
• protein assay standard curve mapper
• 384 well plate layout validation
• technical replicate variance calculator
• laboratory information management system plate mapper
• automated microplate quality control Python client
• ELISA plate design web tool