phytreon 0.1.1

Phylogenetic trees and publication figures in Python

Phylogenetic trees and publication-quality figures in Python.

phytreon combines tree inference, metadata-aware visualization, and static/interactive figure export in a fluent, pure-Python workflow.

Why phytreon?

🌿 Fluent TreeFigure builder Compose a figure by chaining visual layers onto a tree.	🎨 Static + interactive backends matplotlib for PDF/SVG/PNG, plotly for interactive HTML.	🧬 Sequence-to-tree pipeline One call: align → trim → infer → bootstrap.
🧫 Metadata rings / heatmaps / tracks Annotate tips with rings, heatmaps, bars, alignments.	🌳 ML / parsimony / NJ inference Pure-Python likelihood, parsimony, and distance trees.	📄 Publication-ready exports Vector PDF/SVG, raster PNG, or interactive HTML.

A circular 16S rRNA tree of common microbes (real NCBI data bundled in examples/), with domain / phylum / length rings — built and drawn entirely in phytreon.

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Quickstart

pip install -e .                 # core (numpy, scipy, pandas, matplotlib, biopython)
pip install -e .[interactive]    # + plotly (interactive HTML backend)
pip install -e .[dev]            # + pytest, plotly

import phytreon as pt

tr = pt.datasets.primates()                      # a small illustrative toy tree
meta = pt.datasets.primates_metadata().reset_index()
tr.join_data(meta, on="name")                    # attach metadata to tips

(pt.TreeFigure(tr)                               # skeleton drawn for you
    .tip_points(color="habitat", size=9)         # color mapped from metadata
    .tip_labels()
    .support_labels()                            # node support values
).save("tree.pdf")                               # PDF/SVG/PNG -> matplotlib
 # .save("tree.html")                            # HTML        -> plotly (zoom/hover)

The output backend is chosen from the file extension: .pdf / .svg / .png render through matplotlib, .html renders an interactive plotly figure.

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Gallery

Every figure below is produced by a script in examples/ (regenerate with python examples/<name>.py).

Rectangular 16S tree of life, tips colored by domain, bootstrap support (200 reps) — tree_of_life_demo.py	Annotated circular Lineage-colored branches, shaped tips, tile + bar rings, three legends — showcase_circular.py
Aligned tracks Stacked categorical tile tracks plus a numeric bar track — tracks_demo.py	Alignment track The multiple-sequence alignment as a residue raster — tracks_demo.py

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From sequences to a tree

One configurable call runs align → trim → infer → bootstrap, each stage opt-in and fully parameterized:

tree = pt.build_tree(
    "seqs.fasta",                       # path, list of (name, seq), or Alignment
    aligner="builtin",                  # pure-Python MSA  (or "mafft"/"muscle"/"none")
    align_kw=dict(match=2, gap=-3),
    trim_kw=dict(max_gap=0.4, min_occupancy=0.5, min_conservation=0.3),
    method="nj",                        # "nj" | "upgma" | "ml" | "parsimony"
    root="midpoint",
    bootstrap=200,                      # bipartition support
)
# distances are JC69-corrected by default (dist_model="jc69"|"k2p"|"raw");
# negative NJ branch lengths are clamped to 0.

# maximum likelihood (pure Python), HKY85 + Γ4 rate variation + NNI:
ml = pt.build_tree("seqs.fasta", method="ml", ml_model="HKY85", ml_gamma=4,
                   bootstrap=100)               # bootstrap works for nj/ml/parsimony
print(ml.data["logL"], ml.data["AIC"], ml.data["gamma_shape"])
pt.model_finder("seqs.fasta")                   # rank JC/K80/HKY/GTR ±G by AIC
# large datasets -> external engines: build_tree(..., method="ml", ml_engine="iqtree")

Each step is also usable on its own: pt.align, pt.trim, pt.neighbor_joining, pt.bootstrap_support, pt.infer_ml, pt.parsimony_tree.

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What phytreon includes

Area	Capabilities
I/O & data model	Newick / Nexus / PhyloXML read-write; metadata joins (Tree.join_data)
Layouts	rectangular, slanted, dendrogram, circular, fan, radial, inward-circular, unrooted (equal-angle / equal-daylight)
Inference	NJ, UPGMA (model-corrected distances), ML (JC69/K80/HKY85/GTR, +Γ, NNI, AIC/BIC), parsimony, bootstrap, built-in MSA, trimming
Comparative	ancestral states (parsimony / Mk-ML ER·SYM·ARD / Brownian), stochastic mapping, painted branches, node pies
Figure tracks	tip / node / support labels, tip points, metadata rings, heatmaps, bar tracks, alignment rasters
Tree operations	rotate, flip, ladderize, collapse, scale clade, midpoint root, cut tree, Robinson-Foulds

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The TreeFigure builder

TreeFigure starts from a tree skeleton and lets you compose visual layers fluently — every method returns the figure, so calls chain.

Method	Draws
.branches(color=, size=)	the tree skeleton (e.g. color by lineage)
.tip_labels() / .node_labels() / .support_labels()	text labels
.tip_points() / .node_points() / .points()	markers (color / size / shape mapping)
.highlight(node=) / .clade_label(...)	shade / bracket a clade
.heatmap(df)	a matrix of cells aligned to the tips (rectangular)
.ring(df, columns=…)	concentric metadata rings (circular), tile or bar
.bar_track(df, col)	a horizontal bar track
.alignment(aln)	a residue-colored MSA raster
.painted_branches()	branches painted by stochastic-map state
.node_pies()	ancestral-state pies at internal nodes
.time_axis(geo=True)	a time / geological-period axis

Continuous columns get a colorbar, categorical ones a legend; tracks, labels, and legends are placed so nothing overlaps. Layouts: rectangular, slanted, dendrogram, circular, fan, radial, inward_circular, unrooted / daylight (equal-daylight), equal_angle.

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Architecture

The single design decision that makes the dual backend work: layout and rendering are completely decoupled. A layout computes final cartesian coordinates and emits backend-agnostic primitives; matplotlib and plotly are "dumb" renderers that only translate those primitives. Adding a backend means writing one translator — nothing in the phylogenetic logic changes.

Module	Responsibility
core	Tree / Node data model + I/O
layout	topology → display coordinates
scene	Path / Marker / Label / Polygon primitives
plot	TreeFigure builder + matplotlib / plotly backends
infer	alignment / trimming / NJ / ML / parsimony / bootstrap
comparative	ancestral states + stochastic mapping

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Comparison to other Python tools

	phytreon	ete3	toytree	Bio.Phylo	dendropy
Fluent figure builder	✅	✗	partial	✗	✗
Static and interactive backend	✅ mpl + plotly	own GUI / SVG	toyplot	basic mpl	✗
Annotation tracks (heatmap / rings / MSA / bars)	✅	partial	partial	✗	✗
Built-in ML (+Γ) / parsimony	✅ pure-Python	✗	✗	✗	✗
Comparative (ancestral states / stochastic map)	✅	✗	✗	✗	partial
Pure Python, pip-installable	✅	✅ (Qt for GUI)	✅	✅	✅

phytreon's niche is a fluent figure builder plus a self-contained phylogenetics stack, with optional external tools for large-scale alignments or rigorous large-scale ML (aligner="mafft", ml_engine="iqtree").

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Validation

validation/validate.py checks the core algorithms in pure Python (no external tools):

• the likelihood engine (pattern-compressed, rescaled) matches an independent naive re-implementation to machine precision (|Δ| ≈ 1e-13);
• neighbor-joining recovers a tree exactly from its own additive (patristic) distances (Robinson-Foulds = 0, via pt.robinson_foulds) — the defining guarantee of NJ;
• ML recovers a known clade and reports a finite logL / AIC.

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More

Reshaping trees (move branches freely)

pt.ladderize(tree)                      # tidy ordering
pt.rotate(tree, node)                   # flip a clade's vertical order
pt.flip(tree, node_a, node_b)           # swap two clades' positions
pt.collapse_low_support(tree, 70)       # weak edges -> polytomies
pt.scale_clade(tree, node, 0.5)         # de-emphasize a clade's branch lengths
pt.midpoint_root(tree)                  # root an unrooted (NJ) tree
clusters = pt.cut_tree(tree, k=4)       # {tip_name: cluster_id}

Because layout derives tip rows from child order, rotate / flip are exactly how you nudge branches up and down on the plot.

Comparative & time-scaled trees

pt.stochastic_map(tree, trait, n=200)        # stochastic character mapping
(pt.TreeFigure(tree).painted_branches()      # branches painted by inferred state
    .tip_labels())

(pt.TreeFigure(dated_tree)                    # branch lengths = time
    .time_axis(geo=True, gridlines=True, unit="Mya")  # geological bands
    .tip_labels())

Circular tree with metadata rings

(pt.TreeFigure(tree, layout="circular", extent=320)
    .ring(meta_df,                             # DataFrame indexed by tip name
          columns=["habitat", "diet", "body_mass_kg"],
          width=0.13, gap=0.03)                # each column -> one ring
    .tip_labels()
).save("rings.png")

Rings stack outward (categorical → palette, numeric → gradient, each with its own legend); tip labels are pushed outside all rings automatically, so the tree, rings, labels, and legends never overlap.

Colors

Categorical aesthetics use an HCL hue-wheel palette by default (for 3 levels: a balanced #F8766D #00BA38 #619CFF); continuous aesthetics use a dark-blue → light-blue gradient. Override per element:

fig.tip_points(color="habitat", palette="dark2")   # hue | set2 | dark2 | tab10
fig.heatmap(mat, cmap="viridis")                    # name, or ("#fff","#c00") gradient

See phytreon/plot/palettes.py (hue_palette, lerp_color).

Environment notes

• Static figures use matplotlib (.save("x.pdf"/".png"/".svg")) and are always reliable. Interactive output uses plotly (.save("x.html")).
• plotly's static PNG export (kaleido) is flaky on some Windows setups; pin kaleido==0.2.1 for plotly 5, or just use matplotlib for static figures and HTML for interactivity.
• The plotly backend's legends are click-to-toggle traces and track placement is heuristic (it cannot measure text); the matplotlib backend is the reference for exact, publication-quality layout.

Caveats

• The built-in aligner is a single-pass progressive aligner (linear gaps); fine for small/medium inputs, but use MAFFT (aligner="mafft") for publication alignments.
• Native ML / parsimony assume binary trees; NNI search skips polytomies (resolve them first if needed). Pure-Python ML / MSA target tens of taxa — see benchmark/.

Extending

• New layout: subclass phytreon.layout.base.Layout, implement compute() (write node.x / node.y), branch_path(), child_connector(), and register it in phytreon/layout/__init__.py::LAYOUTS. The builder and both backends pick it up automatically.
• New element: subclass phytreon.plot.figure._Element, implement apply(ctx) to read coordinates and append scene primitives (use ctx.resolve_color(...) for metadata-driven aesthetics + legends), then add it with TreeFigure.add(...).

Example data (real, from NCBI)

examples/data/ ships a small microbial "tree of life" 16S rRNA set downloaded from NCBI — common model organisms and type strains across the major bacterial phyla plus four archaea (a natural outgroup) — so the whole pipeline runs on real, public sequences:

examples/data/tol_16S.fasta          18 unaligned 16S sequences (mostly RefSeq NR_*)
examples/data/tol_16S_aligned.fasta  built-in MSA of the above (cached)
examples/data/tol_metadata.csv       domain / phylum / organism / accession / length
examples/data/fetch_example_data.py  re-download script (Entrez)

An NJ/ML tree on this set recovers the four archaea as a monophyletic clade (deep bacterial splits from a single 16S gene are, as expected, only weakly supported). Accession versions may advance over time, so re-downloading need not byte-match the shipped snapshot; the cached alignment keeps the examples reproducible offline. Full accession list and license: examples/data/SOURCES.md.

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Examples & tests

python examples/demo.py               # rect / circular / heatmap / nj / ancestral
python examples/pipeline_demo.py      # raw sequences -> align -> trim -> NJ -> bootstrap
python examples/tree_of_life_demo.py  # real 16S -> tree + circular metadata rings
python examples/showcase_circular.py  # lineage colors + tile + bar rings + shapes
python examples/tracks_demo.py        # rectangular tile / bar tracks + alignment track
python examples/ml_demo.py            # native pure-Python ML tree (HKY85)
python validation/validate.py         # pure-Python correctness checks
python benchmark/benchmark.py         # timings + validated-core guidance
pytest -q                             # 37 tests

# docs: pip install mkdocs-material mkdocstrings[python]; mkdocs serve

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_{MIT licensed · Built for reproducible phylogenetic visualization in Python.}