Python wrappers for PICSL GreedyReg Image Registration Tool

This project provides a Python interface for the Greedy tool from the Penn Image Computing and Science Laboratory, developers of ITK-SNAP.

Greedy is a fast tool for affine and deformable registration of 3D (and 2D) medical images. Please see Greedy Documentation for complete documentation.

This project makes it possible to interface with Greedy from Python code. You can execute registration pipelines as you would on the command line, and you pass SimpleITK image objects to and from Greedy as inputs or outputs.

Quick Start

Install the package:

pip install picsl_greedy

Download a pair of images and a binary mask to experiment with

DATAURL=https://github.com/pyushkevich/greedy/raw/master/testing/data
curl -L $DATAURL/phantom01_fixed.nii.gz -o phantom01_fixed.nii.gz
curl -L $DATAURL/phantom01_moving.nii.gz -o phantom01_moving.nii.gz
curl -L $DATAURL/phantom01_mask.nii.gz -o phantom01_mask.nii.gz

Perform rigid registration in Python

from picsl_greedy import Greedy3D

g = Greedy3D()

# Perform rigid registration
g.execute('-i phantom01_fixed.nii.gz phantom01_moving.nii.gz '
          '-gm phantom01_mask.nii.gz '
          '-a -dof 6 -n 40x10 -m NMI '
          '-o phantom01_rigid.mat')
          
# Apply rigid transform to moving image
g.execute('-rf phantom01_fixed.nii.gz '
          '-rm phantom01_moving.nii.gz phantom01_resliced.nii.gz '
          '-r phantom01_rigid.mat')

SimpleITK Interface

You can read/write images from disk using the command line options passed to the execute command. But if you want to mix Python-based image processing pipelines and Greedy pipelines, using the disk to store images creates unnecessary overhead. Instead, it is possible to pass SimpleITK to Greedy as image inputs and outputs. It is also possible to pass NumPy arrays as rigid and affine transformations.

To pass objects already available in your Python environment, you can use arbitrary strings instead of filenames in the Greedy command, and then use keyword arguments to associate these strings with SimpleITK images or NumPy arrays:

img_fixed = sitk.ReadImage('phantom01_fixed.nii.gz')
img_moving = sitk.ReadImage('phantom01_moving.nii.gz')
g.execute('-i my_fixed my_moving '
          '-a -dof 6 -n 40x10 -m NMI '
          '-o phantom01_rigid.mat', 
          my_fixed = img_fixed, my_moving = img_moving)

Conversely, to retrieve an image or transform output by Greedy into the Python envirnonment, you can also replace the filename by a string, and use keyword arguments to assign None to that string. You can then retrieve the output using the [] operator:

g.execute('-i phantom01_fixed.nii.gz phantom01_moving.nii.gz '
          '-gm phantom01_mask.nii.gz '
          '-a -dof 6 -n 40x10 -m NMI '
          '-o my_rigid',
          my_rigid=None)
          
mat_rigid = g['my_rigid']

The example below performs affine and deformable registration and then applies reslicing to the moving image without having Greedy write any files to disk.

from picsl_greedy import Greedy3D
import SimpleITK as sitk
import numpy as np

# Load the images
img_fixed = sitk.ReadImage('phantom01_fixed.nii.gz')
img_moving = sitk.ReadImage('phantom01_moving.nii.gz')
img_mask = sitk.ReadImage('phantom01_mask.nii.gz')

g = Greedy3D()

# Perform affine registration
g.execute('-i my_fixed my_moving '
          '-a -dof 6 -n 40x10 -m NMI '
          '-o my_affine',
          my_fixed = img_fixed, my_moving = img_moving, my_mask = img_mask,
          my_affine = None)

# Report the determinant of the affine transform
print('The affine transform determinant is ', np.linalg.det(g['my_affine']))

# Perform deformable registration
g.execute('-i my_fixed my_moving '
          '-it my_affine -n 40x10 -m NCC 2x2x2 -s 2.0vox 0.5vox '
          '-o my_warp',
          my_warp = None)

# Apply the transforms to the moving image
g.execute('-rf my_fixed -rm my_moving my_resliced '
          '-r my_warp my_affine',
          my_resliced = None)

# Save the resliced image
sitk.WriteImage(g['my_resliced'], 'phantom01_warped.nii.gz')

Metric Log

If you would like to access the optimization metric value across iterations, use the method metric_log().

from picsl_greedy import Greedy3D
import SimpleITK as sitk
import numpy as np
import matplotlib.pyplot as plt

# Load the images
img_fixed = sitk.ReadImage('phantom01_fixed.nii.gz')
img_moving = sitk.ReadImage('phantom01_moving.nii.gz')
img_mask = sitk.ReadImage('phantom01_mask.nii.gz')

g = Greedy3D()

# Perform affine registration
g.execute('-i my_fixed my_moving '
          '-a -dof 6 -n 40x10 -m NMI '
          '-o my_affine',
          my_fixed = img_fixed, my_moving = img_moving, my_mask = img_mask,
          my_affine = None)

# Report metric value
ml = g.metric_log()
plt.plot(ml[0]["TotalPerPixelMetric"], label='Coarse')
plt.plot(ml[1]["TotalPerPixelMetric"], label='Fine')
plt.legend()
plt.title('Metric value')
plt.savefig('metric.png')

Propagation

The Propagation tool warps a 3D segmentation from a reference time point to all target time points in a 4D image. This is useful for generating 4D segmentation from sparse 3D segmentation.

Basic Usage (Files on Disk)

from picsl_greedy import Propagation

p = Propagation()

# Propagate segmentation from time point 5 to other time points
p.run('-i img4d.nii.gz '
      '-sr3 seg_tp05.nii.gz '
      '-tpr 5 '
      '-tpt 1,2,3,4,6,7,8,9 '
      '-o /path/to/output '
      '-n 100x100 -m SSD -s 3mm 1.5mm')

Using SimpleITK Images

You can pass SimpleITK images directly to avoid disk I/O overhead. Use arbitrary strings as placeholders and pass the actual images as keyword arguments:

from picsl_greedy import Propagation
import SimpleITK as sitk

# Load images
img4d = sitk.ReadImage('img4d.nii.gz')
seg3d = sitk.ReadImage('seg_tp05.nii.gz')

p = Propagation()

# Run propagation with in-memory images
p.run('-i my_img4d '
      '-sr3 my_seg3d '
      '-tpr 5 '
      '-tpt 1,2,3,4,6,7,8,9 '
      '-n 100x100 -m SSD -s 3mm 1.5mm',
      my_img4d=img4d, my_seg3d=seg3d)

# Retrieve 4D segmentation output
seg4d_out = p['seg4d']
sitk.WriteImage(seg4d_out, 'seg4d_propagated.nii.gz')

# Retrieve 3D segmentation for a specific time point
seg_tp03 = p['seg_03']
sitk.WriteImage(seg_tp03, 'seg_tp03.nii.gz')

# Get list of processed time points
time_points = p.get_time_points()
print(f'Processed time points: {time_points}')

Using 4D Segmentation Input

If your reference segmentation is already embedded in a 4D image, use -sr4 instead of -sr3:

from picsl_greedy import Propagation
import SimpleITK as sitk

img4d = sitk.ReadImage('img4d.nii.gz')
seg4d_sparse = sitk.ReadImage('seg4d_sparse.nii.gz')  # Only time point 5 has segmentation

p = Propagation()

p.run('-i my_img4d '
      '-sr4 my_seg4d '
      '-tpr 5 '
      '-tpt 1,2,3,4,6,7,8,9 '
      '-n 100x100 -m SSD',
      my_img4d=img4d, my_seg4d=seg4d_sparse)

# Get complete 4D segmentation
seg4d_complete = p['seg4d']

Command Line Options

Option	Description
`-i <image>`	4D input image
`-sr3 <image>`	3D reference segmentation at reference time point
`-sr4 <image>`	4D reference segmentation (only reference time point used)
`-tpr <int>`	Reference time point
`-tpt <list>`	Target time points (comma-separated)
`-o <dir>`	Output directory (for file-based output)
`-sr-op <pattern>`	Output filename pattern, e.g., `Seg_%02d.nii.gz`
`-n <schedule>`	Multi-resolution schedule (default: `100x100`)
`-m <metric>`	Registration metric: `SSD`, `NCC`, `NMI` (default: `SSD`)
`-s <sigmas>`	Smoothing kernels (default: `3mm 1.5mm`)
`-dof <int>`	Affine degrees of freedom (default: `12`)
`-threads <int>`	Number of threads
`-V <0\|1\|2>`	Verbosity level

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picsl-greedy 1.4.0

Python wrappers for PICSL GreedyReg Image Registration Tool

Quick Start

SimpleITK Interface

Metric Log

Propagation

Basic Usage (Files on Disk)

Using SimpleITK Images

Using 4D Segmentation Input

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Algorithm	Hash digest
SHA256	`e4d3ab6347e40bac53b6e875f8c1f20cdf5611ca6bd93fb7c9b095c596303952`
MD5	`a447dcf7291a430d1202fba50fda0907`
BLAKE2b-256	`a92e12d700dfc453c2d86796b94f54c0bf898dcec714a01150fd89248c3e9543`

Algorithm	Hash digest
SHA256	`eb7f360bb96eccb8b144fa45ee0f0e64286d0beeb15614cf3c91052da9ad7f09`
MD5	`9d57f02ec70159de915479f4cf3e033e`
BLAKE2b-256	`c0b893dba8f112cc162baf62cc41ff3dcab20438cca24daecefc7507fa13e9eb`

Algorithm	Hash digest
SHA256	`3d274d756c8fc66e3a8e15d816c871491e33aa9f1f999ba28d3f9c128f7548d0`
MD5	`613255677aac57cf40ab17e496ac3f6b`
BLAKE2b-256	`d04a70041890ba6b6a8cd13b5aa6d044dcf5237d34a8b119fefc57a423e29f5e`

Algorithm	Hash digest
SHA256	`4883a453b2c3b3482778421f381fad632d7e28a275cbb2838feea2c798062ab7`
MD5	`1a3f7b2f15bd0952b218267cdf4f8dd4`
BLAKE2b-256	`2ca892a407ac63cc4fe178e51761d3f18d20c97c364e73cf77515e54abd70b89`

Algorithm	Hash digest
SHA256	`eb404ca9f5f25c3119007539432434363ae2d6b29f1bd552be22b3c68d617799`
MD5	`1be2ae3ae1e1c6f9ea19ec1daa04b18c`
BLAKE2b-256	`83c109d9fabe2c8eb36aa43afa95f187071758bb3f39b3c995b50aab127a5e78`

Algorithm	Hash digest
SHA256	`658ffdc283fbc27f513bcfca6410a8a3a4372e4d55dc6e08299a44294585c6f6`
MD5	`7929fbae8f992072be700855119ac4cc`
BLAKE2b-256	`707e692666c35d0dedf18300d919be2c1bcf3f54afb4ac93bd27287fa433ebba`

Algorithm	Hash digest
SHA256	`b693a0dd532c5c63b6a03cc295786ab07327fd793b5750dfa603513ca9f63336`
MD5	`2c655c33029bfdf42567165b32eae635`
BLAKE2b-256	`66a2c7413644405658395f5a81a688b257cfcae65d3e145e7074a7da338cd1b6`

Algorithm	Hash digest
SHA256	`d25fb5116402b6a454444f22a1e0953c9f44a074765791f3b69f00b0dcff2532`
MD5	`0268a8b60fee6f48cba04b0393ebb6b0`
BLAKE2b-256	`7b13e87f94619f7ef9c84d48b99adc2b39ebd5ee7d86c098456a1f150fc6279c`

Algorithm	Hash digest
SHA256	`ebcf5076ab379dd6709ebd4882720050379a345d5d2e106fdc8ec7576f0a6d10`
MD5	`f63e04cf36196ac2c1f0be4df807adb9`
BLAKE2b-256	`448ef78cb91d769466cb9dc1a8e5c098181013c4b31e7e4b44df7741d0fab6df`

Algorithm	Hash digest
SHA256	`096517799fc89f023fdce0817c7331814cefe75574e617e951e1264e5b0bca84`
MD5	`23f9b2eac2c7ad9f7ab176d472aea2c2`
BLAKE2b-256	`63588b0b0cd5d39fa8c5e257d73d5de08bb0da766e5e86bf85d6e4ae7902eb9c`