Analyse a genome for CRISPRs and off-targets
Project description
Python CRISPR Analyser
Introduction
The Python CRISPR Analyser is a set of libraries and scripts that allows you to index CRISPRs from FASTA genome files and use the resulting index to find CRISPRS by using guide RNA (gRNA) sequences and to find off-targets for a given gRNA sequence. This code is a re-write of the original CRISPR Analyser written in C/C++ with the intention that the Python version will be easier to use and extend.
For the efficient search for CRISPR off-targets, Python CRISPR Analyser supports CUDA on Linux platforms.
py-crispr-analyser uses Python 3.10 or later and is tested on Linux and MacOS. It has not been tested on Windows.
Documentation
All documentation is online at https://py-crispr-analyser.readthedocs.io/.
Copyright
Copyright (C) 2025-2026 Genome Research Ltd.
License
Distributed under the MIT License. See LICENSE for more information.
Contact
Cellular Informatics Team at the Wellcome Sanger Institute, Hinxton, UK - wge@sanger.ac.uk
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