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Project Description

pysickle.py will try to remove sequences that cause misalignments from a multiple sequence alignment (MSA). It reads a given MSA in multi-fasta format and removes sequences with the highest penalty scores, then builds the next MSA without those sequences. This process is repeated until a user-specified cuttoff is reached or less than three sequences are left to be aligned.

Usage:

######################################
# pysickle.py
######################################
usage:
    pysickle.py -f multifasta alignment
options:
    -f, --fasta=FILE    multifasta alignment (eg "align.fas")
    OR
    -F, --fasta_dir=DIR directory with multifasta files (needs -s SUFFIX)
    -s, --suffix=SUFFIX will try to work with files that end with SUFFIX
                        (eg ".fas")

    -a, --msa_tool=STR  supported: "mafft" [default:"mafft"]
    -i, --max_iterations=NUM    force stop after NUM iterations
    -n, --num_threads=NUM   max number of threads to be executed in
                            parallel [default: 1]

    -h, --help      prints this

Currently supported multiple sequence aligners:

  • mafft (Katoh, Standley 2013 (Molecular Biology and Evolution 30:772-780) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. http://mafft.cbrc.jp/alignment/software/)

Requirements

  • matplotlib
  • numpy

External Programs

  • mafft
Release History

Release History

0.1.1

This version

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0.1

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Download Files

Download Files

TODO: Brief introduction on what you do with files - including link to relevant help section.

File Name & Checksum SHA256 Checksum Help Version File Type Upload Date
pysickle-0.1.1-py2.7.egg (24.1 kB) Copy SHA256 Checksum SHA256 2.7 Egg May 21, 2014
pysickle-0.1.1.tar.gz (20.6 kB) Copy SHA256 Checksum SHA256 Source May 21, 2014

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