Time-correlated single photon counting (TCSPC) data analysis

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  • License: MIT License (MIT License Copyright (c) 2022 Easun Arunachalam Permission is hereby granted, free of charge, to ...)
  • Author: Easun Arunachalam
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Project description

pytcspc: a Python library for fluorescence lifetime imaging microscopy (FLIM) and fluorescence correlation spectroscopy (FCS) data analysis

Installing

Please see INSTALLATION.md.

Functions

FLIM

  • read Becker & Hickl .sdt files (based on sdtfile) into user-friendly xarray.DataArrays suitable for further analysis
  • produce intensity and lifetime images
  • fit decay curves to multiexponential models using least-squares or Gibbs sampling approaches

FCS

  • read Becker & Hickl .spc files into user-friendly xarray.DataArrays suitable for further analysis
  • generate FLIM and intensity images and "videos"
  • generate kymographs for line-scanning FCS
  • calculate correlation functions (based on multipletau)

examples

  • FCS: fit FCS data for diffusion of Alexa Fluor 488
  • fit_oneexp: fit decay curve for a solution of FAD
  • fit_from_image: fit decay curve for NAD(P)H in yeast

Contributing

Please see CONTRIBUTING.md.

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  • License: MIT License (MIT License Copyright (c) 2022 Easun Arunachalam Permission is hereby granted, free of charge, to ...)
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  • Tags example , setuptools
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