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A easy-to-use Hi-C processing software supporting distributed computation

Project description

https://zenodo.org/badge/doi/10.5281/zenodo.55324.svg

runHiC is an easy-to-use command-line tool for Hi-C data processing.

Since version 0.8.1, runHiC can be used directly on Arima HiC data by setting the enzyme name to Arima.

Since version 0.8.0, runHiC has changed its default data container/format from HDF5 to Pairs and Cooler. (See Release Notes)

Design Concepts

runHiC is designed to process Hi-C data from raw sequencing reads(.sra, .fastq, .fastq.gz) to the corrected contact matrices. It currently contains 5 subcommand:

mapping Map raw pair-end sequencing data to a supplied genome. Support bwa and minimap2.
filtering Perform read-level and fragment-level noise removing
binning 1.Generate contact matirx; 2. Perform ICE
pileup Perform entire processing from mapping to binning
quality Assess the quality of your Hi-C data

Usage

Open a terminal, type runHiC -h and runHiC <subcommand> -h for help information.

Citation

Xiaotao Wang. (2016). runHiC: A user-friendly Hi-C data processing software based on hiclib. Zenodo. 10.5281/zenodo.55324

Project details


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