Skip to main content

scprep

Project description

scprep logo Latest PyPi version Latest Conda version Travis CI Build Read the Docs Coverage Status Twitter GitHub stars

scprep provides an all-in-one framework for loading, preprocessing, and plotting matrices in Python, with a focus on single-cell genomics.

The philosophy of scprep:

  • Data shouldn’t be hidden in a complex and bespoke class object. scprep works with numpy arrays, pandas data frames, and scipy sparse matrices, all of which are popular data formats in Python and accepted as input to most common algorithms.
  • Your analysis pipeline shouldn’t have to change based on data format. Changing from a numpy array to a pandas data frame introduces endless technical differences (e.g. in indexing matrices). scprep provides data-agnostic methods that work the same way on all formats.
  • Simple analysis should mean simple code. scprep takes care of annoying edge cases and sets nice defaults so you don’t have to.
  • Using a framework shouldn’t be limiting. Because nothing is hidden from you, you have access to the power of numpy, scipy, pandas and matplotlib just as you would if you used them directly.

Installation

preprocessing is available on pip. Install by running the following in a terminal:

pip install --user scprep

Alternatively, scprep can be installed using Conda (most easily obtained via the Miniconda Python distribution):

conda install -c bioconda scprep

Quick Start

You can use scprep with your single cell data as follows:

import scprep
# Load data
data_path = "~/mydata/my_10X_data"
data = scprep.io.load_10X(data_path)
# Remove empty columns and rows
data = scprep.filter.remove_empty_cells(data)
data = scprep.filter.remove_empty_genes(data)
# Filter by library size to remove background
scprep.plot.plot_library_size(data, cutoff=500)
data = scprep.filter.filter_library_size(data, cutoff=500)
# Filter by mitochondrial expression to remove dead cells
mt_genes = scprep.select.get_gene_set(data, starts_with="MT")
scprep.plot.plot_gene_set_expression(data, genes=mt_genes, percentile=90)
data = scprep.filter.filter_gene_set_expression(data, genes=mt_genes,
                                                percentile=90)
# Library size normalize
data = scprep.normalize.library_size_normalize(data)
# Square root transform
data = scprep.transform.sqrt(data)

Help

If you have any questions or require assistance using scprep, please read the documentation at https://scprep.readthedocs.io/ or contact us at https://krishnaswamylab.org/get-help

Project details


Download files

Download the file for your platform. If you're not sure which to choose, learn more about installing packages.

Files for scprep, version 1.0.3
Filename, size File type Python version Upload date Hashes
Filename, size scprep-1.0.3-py3-none-any.whl (96.0 kB) File type Wheel Python version py3 Upload date Hashes View hashes
Filename, size scprep-1.0.3.tar.gz (91.8 kB) File type Source Python version None Upload date Hashes View hashes

Supported by

Elastic Elastic Search Pingdom Pingdom Monitoring Google Google BigQuery Sentry Sentry Error logging AWS AWS Cloud computing DataDog DataDog Monitoring Fastly Fastly CDN DigiCert DigiCert EV certificate StatusPage StatusPage Status page