SMAPpy
SMAPpy is software for single-molecule localization microscopy (SMLM) in Python. It takes raw camera frames to localizations and super-resolution images, with a graphical interface, command-line tools and a Python API. It is a Python port of SMAP.
Install
pip install smappy-smlm
The GUI is included. Binary wheels are available for Python 3.12-3.14 on macOS,
Linux and Windows, so nothing needs to be compiled. The package is called
smappy-smlm on PyPI; in Python you import smappy.
I need more help. Never used a terminal or pip?
Installing SMAPpy, step by step walks through
it on a Mac or Windows computer, from opening the terminal to starting the GUI,
and says what to ask an AI assistant that can do it for you.
With uv you can install the GUI as a standalone tool, or run it without installing:
uv tool install smappy-smlm
uvx --from smappy-smlm smappy-gui
To install from a checkout, which builds the C++ extensions (pip 21.3 or newer):
git clone https://github.com/ries-lab/SMAPpy.git
cd SMAPpy
python -m venv .venv
.venv/bin/python -m pip install -e .
Update
Close SMAPpy first: on Windows, pip cannot replace the program files of a running SMAPpy. Then, in the same environment you installed it into:
pip install --upgrade smappy-smlm
pip show smappy-smlm tells you which version you have; the newest is on
PyPI. If you installed it with uv,
run uv tool upgrade smappy-smlm instead. In a checkout, run git pull, then
.venv/bin/python -m pip install -e . again, which rebuilds the C++
extensions.
SMAPpy itself looks for a newer version once a day when it starts and, if there is one, says so at the bottom right of the control window. Help → Check for updates... asks at any time. Its Update and restart closes SMAPpy, installs the new version with pip (or uv) and starts SMAPpy again; Copy command gives the command for your installation instead, and a checkout is always updated by hand. The daily check is turned off in the General tab of Preferences....
Start
smappy-gui
or, to open a localization file directly:
smappy-gui FILE.hdf5
Files can also be dropped onto either window: a localization file opens as
with File → Open; a short TIFF (50 frames or fewer) or a PNG is added as an
image layer, as with File → Open image; and a longer stack (TIFF, an NDTiff
folder, *.sim.yaml) becomes the file of the fitter on the Localize tab,
ready to fit.
From a checkout, activate the venv first or run .venv/bin/smappy-gui.
Tutorials and documentation
Tutorials. ries-lab.github.io/SMAPpy has short guided tours on simulated data you can follow along with. Start with SMAPpy in three minutes.
Help pages. Every plugin and the main parts of the GUI have a help page.
It explains the theoretical background and every control. Open it with the
? in a plugin's or a Render-tab section's title bar, with F1 in any panel or
window, or from Help → Plugin documentation. The same pages are online at
ries-lab.github.io/SMAPpy/help, and
python -m smappy.docs -o DIR writes them as a static website.
Your own analysis. When no plugin measures what you need, make one: describe it to an AI assistant started in your plugin folder (Plugins → Show my plugin folder), or start from the example with Plugins → New plugin.... A file saved there appears under Plugins → User. The help page Your own plugins explains how.
For developers. GUI.md describes the architecture. docs/ holds design notes on the plugin system and individual algorithms. NOTES.md records design decisions and measurements.
License
SMAPpy is released under the BSD 3-Clause license; see LICENSE and THIRD_PARTY_NOTICES.md.
1 maintainer
Metadata
1 maintainer
Release files for smappy-smlm 0.5.1
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Built distributions (wheels)
Total release size: 37.0 MB
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