sopa 2.2.2

Spatial-omics pipeline and analysis

Spatial omics pipeline and analysis

Built on top of SpatialData, Sopa enables processing and analyses of spatial omics data with single-cell resolution (spatial transcriptomics or multiplex imaging data) using a standard data structure and output. We currently support the following technologies: Xenium, Visium HD, MERSCOPE, CosMx, PhenoCycler, MACSima, Molecular Cartography, and others. Sopa was designed for generability and low memory consumption on large images (scales to 1TB+ images).

[!NOTE] You may also be interested in Novae, developed by the same authors, now published in Nature Methods 🎉

Documentation

Check Sopa's documentation to get started. It contains installation explanations, CLI/API details, and tutorials.

Overview

The following illustration describes the main steps of sopa:

Installation

Sopa can be installed from PyPI on all OS, for any Python version >=3.11. On a new environment, run the following command:

pip install sopa

[!NOTE] See this installation section for more details about extras and other installations modes.

Features

Sopa comes in four different flavours, each corresponding to a different use case:

• API: use directly sopa as a Python package for complete flexibility and customization.
• Snakemake pipeline: choose a config, and run our pipeline on your spatial data in a couple of minutes.
• nf-core/sopa: run Sopa with Nextflow (see this repo and the corresponding usage guide). Great for Docker users.
• CLI: use our command-line-interface for prototyping quickly your own pipeline (advanced users).

API

Below is a minimal example of API usage. For a complete API description, please refer to the documentation.

import sopa

sdata = sopa.io.xenium("path/to/data") # reading Xenium data

sopa.make_image_patches(sdata) # creating overlapping patches
sopa.segmentation.cellpose(sdata, "DAPI", diameter=30) # running cellpose segmentation
sopa.aggregate(sdata) # counting the transcripts inside the cells

Snakemake pipeline

Clone our repository, choose a config here (or create your own), and execute our pipeline locally or on a high-performance cluster:

git clone https://github.com/gustaveroussy/sopa.git
cd sopa/workflow
snakemake --configfile=/path/to/yaml_config --config data_path=/path/to/data_directory --cores 1 --use-conda

For more details on snakemake configuration and how to properly setup your environments, please refer to the documentation.

CLI

Below are examples of commands that can be run with the sopa CLI. For a complete description of the CLI, please refer to the documentation.

> sopa --help # show command names and arguments
> sopa convert merscope_directory --technology merscope # read some data
> sopa patchify image merscope_directory.zarr # make patches for low-memory segmentation
> sopa segmentation cellpose merscope_directory.zarr --diameter 60 --channels DAPI # segmentation
> sopa resolve cellpose merscope_directory.zarr # resolve segmentation conflicts at boundaries
> sopa aggregate merscope_directory.zarr --average-intensities # transcripts/channels aggregation
> sopa explorer write merscope_directory.zarr # convert for interactive vizualisation

Cite us

Our article is published in Nature Communications. You can cite Sopa as below:

Blampey, Q., Mulder, K., Gardet, M. et al. Sopa: a technology-invariant pipeline for analyses of image-based spatial omics.
Nat Commun 15, 4981 (2024). https://doi.org/10.1038/s41467-024-48981-z