telotjek 0.0.1

Check assembly ends for Streptomycete telomeres using BLAST

Telotjek

Should you inspect the ends of your Streptomycete assembly? If the linear replicons do not contain any of the telomere clusters described in Faurdal et al. 2025 [1] or the six classes of telomeres described in Algora-Gallardo et al 2021 [2] it might be a good idea.

Telotjek provides a quick and dirty way to approach this check. It runs a BLAST against a representative of the 129 telomere clusters generated in Faurdal et al. 2025 [1] or a representative of the six classes outlined in Algora-Gallardo et al 2021 [2] and reports if it matches any of the classes and if it is present in full or truncated.

If you do not have a match in your Complete assembly consider checking if the reads support it being extended further [1] or if you have a potentially novel telomere class on your hands.

Usage

telotjek.py assembly.fasta

To use the 6 representatives outlined by Algora-Gallardo et al 2021:

telotjek.py assembly.fasta --telo_class

Dependencies

Requires blast

Source:

1. Faurdal D, Booth TJ, Weber T, Jørgensen TS. Tying up loose ends: recovering thousands of missing telomeres from Streptomyces and other Streptomycetaceae genomes. bioRxiv [Preprint]. 2025 Oct 14:2025.10.14.682034. doi:10.1101/2025.10.14.682034.
2. Algora-Gallardo L, Schniete JK, Mark DR, Hunter IS, Herron PR. Bilateral symmetry of linear streptomycete chromosomes. Microb Genom. 2021 Nov;7(11):000692. doi: 10.1099/mgen.0.000692. PMID: 34779763; PMCID: PMC8743542.