TxNova

TxNova finds experimental-group-specific novel intergenic transcripts from bulk RNA-seq BAMs. Treat-recurrent residual splices become locus models. Class, counts, junctions, and bridges are recomputed from the BAM and the annotation.

Step	What it does
Harvest	Treat-recurrent CIGAR `N` junctions missing from the annotation → residual loci
Universe	Annotation + residual models, counted together
Gates	Structure, detection, optional DE
Tables	Treat-specific finals; both-group structure-pass (TPM and shared splice)

Docs: Read the Docs.

What you need

Python 3.10+
Coordinate-sorted, indexed BAMs from STAR or HISAT2 (control and treat)
Genome FASTA + .fai, and a comprehensive gene GTF (mouse default: GENCODE M39 / GRCm39)
A YAML config and a sample sheet (txnova init writes starters)

Install

pip install txnova

Wheels cover Linux and macOS. On Windows use WSL2. Building from source needs a Rust toolchain; see Installation.

First run

txnova init -c config.yaml --samples samples.tsv
# edit paths, strandedness (rf / fr / unstranded), and sample rows
txnova preflight -c config.yaml
txnova run -c config.yaml

What to expect under output_dir:

candidates/candidates.tsv — experimental-group-specific finals
candidates/candidates.unnamed.tsv — structure-pass, also in control by TPM
candidates/candidates.shared.tsv — structure-pass, splice in both groups
report/report.html — start here
quantify/ — full-universe counts, TPM, and DE

The three candidate tables share the structural gates. They answer different questions (induction vs unannotated structure in both groups). They are not a declaration of new genes.

Next: Quickstart · Data preparation · Output reference · FAQ

Status

0.1.x. Pin txnova==0.1.5 in Methods. Changelog.

Citation

For the software, cite the Zenodo DOI above. Pin the installed version in Methods (this tree is txnova==0.1.5). See CITATION.cff.

Li, Z. TxNova. Zenodo. doi:10.5281/zenodo.21970482

PyPI: https://pypi.org/project/txnova/.

License

Software: Apache License 2.0. The packaged mouse hexamer table comes from CPAT (Wang et al. 2013); see docs/license.

Author

Zhao Li (李钊)
Email: leelieber@gmail.com

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0.1.6

Aug 19, 2026

This release

0.1.5

Aug 17, 2026

0.1.1

Aug 16, 2026

txnova 0.1.5

TxNova

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