ugbio-omics 1.3.4

Ultima Genomics bioinfo utils for working with AWS Health-Omics

ugbio_omics

This module includes Python scripts for working with AWS HealthOmics.

Log in with SSH to the desired AWS account before running tools in this package.

List of tools:

1. Compare Cromwell vs. Omics - Process cost and performance on both platforms and save results into comparable CSV files. Also saves metadata and intermediate cost and performance files. Additionally, you can find a plots folder with a variety of HTML plots. Omics cost information is collected using Omics Run Analyzer.

   Run uv run compare_cromwell_omics --help for more details.

2. Compare Omics Runs - Process cost and performance and compare multiple Omics runs. Omics cost information is collected using Omics Run Analyzer.

   Run uv run compare_omics_runs --help for more details.

3. Get Run Logs - Download logs of an Omics run. For FAILED run by default, you'll get the logs of all failed tasks or the run's engine log if there are no failed tasks. For successful runs by default, you'll get all tasks' logs. You can use --task-id to get the log for a specific task.

   Run uv run get_omics_logs --help for more details.

4. Manifest Log - Download and parse the manifest log of an Omics run. The manifest log contains a lot of information about storage usage, CPU and memory usage per task, Docker images, inputs, and general information about the run.

   Run uv run manifest_log --help for more details.

Admin tools (requires admin AWS permissions):

1. Report Run Completion - Manually triggers the OmicsEndRunHandler AWS Lambda to report mongodb on end of omics run/s. Run uv run report_run_completion --help for more details.

Run with Docker

A fast and easy way to run the above-mentioned tools is by running a container:

docker run -v ~/.aws:/root/.aws -e AWS_PROFILE=${AWS_PROFILE} -v <local_output>:<output> 337532070941.dkr.ecr.us-east-1.amazonaws.com/ugbio_omics:latest <tool>

Breakdown:

1. A simple docker run command.

2. -v ~/.aws:/root/.aws -e AWS_PROFILE=${AWS_PROFILE} - this will allow the Docker container to use the SSH profile you are logged into (customer/dev/prod).

   Remember to use alog before running the container.

3. -v <local_output>:<output> - add a volume mapping and use it in the output path of the tool to get the outputs available outside the container.

4. 337532070941.dkr.ecr.us-east-1.amazonaws.com/ugbio_omics:latest - Docker image.

5. <tool> - available tools are:

   • get_omics_logs
   • manifest_log
   • compare_omics_runs
   • compare_cromwell_omics