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A tool for MAG taxonomy assignment using Blastn

Project description

Ekl_mgenomics

A tool for MAG (Metagenome Assembled Genome) taxonomy analysis using Blastn.

Installation

pip install Ekl-mgenomics

Usage

ekl_tax yourgenome.fasta

If IndexError---> Go to that chunks blast file and delte the malfunctioned file and again do [ekl_tax yourgenome.fasta]

Requirements

-cd into your folder containing the genome.fasta file.

  • BLAST+ must be installed on your system
  • Internet connection for remote BLAST and Entrez queries

What it does

  1. Splits your fasta file into 200kb chunks per contig
  2. BLASTs each chunk against NCBI nt database
  3. Assigns taxonomy to each contig by majority vote
  4. Writes OrganismTaxonomy.tsv with full results

Author

Eklavya Kumar

Authors Note

Run the command and go for a walk it might take time.......

Made with ❤️️ and Mr. Deepu's guidance.

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