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Project description

PyPl8

Image analysis package for segmenting images of microbial communities and extracting quantitative features automatically.

Background

This package makes use of the cv2 and sci-kit image libraries.

It was developed in the Dudley lab at Pacific Northwest Research Institute and is currently most useful for analyzing images of rectangularly arrayed patches/colonies on omnitrays photographed against a dark background.

Organization

  • The function in the barcode methods can be used to rename images based on a barcode visible in the image if an excel spreadsheet relating barcode numbers to relevant plate information is provided.

  • The functions in the preprocessing methods are used to crop each image to relevant areas of interest

  • ProcessImages.py contains the main functions for processing a single image or a batch of images

  • Now includes parallel batch processing

  • There are several example images included in the package which can be used for demos

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