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RiboParser

RiboParser is a modular command-line toolkit for comprehensive RNA-seq and Ribo-seq data analysis.

📖 Documentation | 🐞 Issues | 📚 Citation | 📝 License


Overview

RiboParser provides an integrated workflow for RNA-seq and ribosome profiling (Ribo-seq) analysis, including reference preparation, raw data preprocessing, alignment, quantification, quality control, RPF density construction, gene-level analysis, codon-level analysis, SeRP analysis, smORF analysis, and downstream visualization with RiboShiny.

The full documentation is available at:

https://renscq.github.io/RiboParser/

Installation

Install the dependency packages with micromamba.

micromamba install cutadapt bowtie samtools star bedtools subread rsem gffread sra-tools \
 ucsc-genepredtogtf ucsc-gtftogenepred ucsc-gff3togenepred ucsc-bedgraphtobigwig ucsc-bedsort \
 -c bioconda

micromamba install pigz -c conda-forge

Install the RiboParser.

micromamba install riboparser -c rensc

# or

pip install riboparser

Quick test

riboparser -v
riboparser -c
riboparser -d
riboparser -m

Citation

Ren, S., Li, Y. & Zhou, Z.
RiboParser/RiboShiny: An integrated platform for comprehensive analysis and visualization of ribo-seq data.
Journal of Genetics and Genomics (2025).
DOI: 10.1016/j.jgg.2025.04.010

License

GPL-3.0-or-later.

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