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Coppafish, Antibody Staining, and Two-photon alignment: 3D alignment framework for microscopy and in vivo imaging

Project description

CASTalign: Coppafish, Antibody Staining, and Two-photon alignment

CASTalign allows you to register 3D microscopy images to each other and to in vivo two-photon imaging. It is optimised to allow registration between CoppaFISH 3D, antibody staining (e.g., immunofluorescence), and in vivo imaging.

Installation

To install:

pip install castalign

You may also want to install (optional) GPU acceleration with cupy. Depending on the version of CUDA, install one of these:

pip install cupy-cuda13x # For CUDA 13.x pip install cupy-cuda12x # Fox CUDA 12.x

Alternatively, you can install from github:

pip install git+https://github.com/mwshinn/castalign.git

Usage

See the tutorial in the documentation.

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