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Dark matter

(Pure) Python scripts and libraries for bioinformatics.

Overview

Utility scripts

The bin directory contains over 100 utility scripts. These fall into the following categories:

  1. FASTA/FASTQ: general sequence inspection, filtering, and manipulation
  2. DNA/amino-acid translation and codon tools
  3. Pairwise sequence comparison
  4. Multiple-sequence-alignment (MSA) and alignment-position analysis
  5. Consensus-sequence building
  6. Read mapping and SAM/BAM analysis
  7. BLAST/DIAMOND hit processing and alignment panels
  8. Protein/genome SQLite databases and GenBank data
  9. Taxonomy
  10. Phylogenetic trees (Newick)

Library functions

  1. Core reads & sequence I/O
  2. DNA & amino-acid biology
  3. Sequence alignment & comparison
  4. Alignment-result containers, filtering & summarization
  5. BLAST
  6. DIAMOND
  7. SAM/BAM, read mapping & consensus building
  8. Protein/genome databases, GenBank & taxonomy
  9. Phylogenetic trees
  10. Visualization: plotting & HTML
  11. General utilities

Installation

pip install dark-matter

or, using uv:

uv add dark-matter

dark-matter supports Python 3.10 through 3.15.

Documentation

The full documentation is at acorg.github.io/dark-matter, built from the Markdown files below:

  • doc/scripts.md — the command-line scripts in bin, for people who just want to run existing tools.
  • doc/library.md — the dark Python library under src/dark, for developers who want to write their own code against it.
  • doc/reads.md — the central Read class hierarchy.

Developers

git clone https://github.com/acorg/dark-matter.git
cd dark-matter
uv sync

Run the tests with

make pytest

or directly via

uv run pytest

License

MIT. See LICENSE.

Metadata

Release files for dark-matter 7.5.3

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