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Search for guide RNA sites in PCR tags

Project description

Search for guide RNA sites in PCR tags


  • Install the package version through pip pip install gRNAsearch
  • from grnasearch.gRNA import search
  • summary, frames = search(syn="../seq.fa", tag="../tag.csv", save=True, output_dir="~", output_name="gRNA")


search(syn, tag, save=True, output_dir='~', output_name="gRNA"):

  • syn: full path to sequence file in '.fa' format
  • tag: PCR tag file
  • save: save the outputs in csv (default=True)
  • output_dir: output directory (full path) to save the files (default='~')
  • output_name: name of the output file (default="gRNA")


  • summary: the search results data frame
  • frames: the intermediate data frame with WT sequences

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