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A command-line tool for retrieving GenBank metadata and mapping coding sequences between query and subject FASTA files to identify their most similar counterparts.

Project description

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A command-line tool for retrieving GenBank metadata and mapping coding sequences between query and subject FASTA files to identify their most similar counterparts.

Features

  • Automated retrieval of GenBank metadata for query and reference sequences files in FASTA format.

  • Parallelized mapping of query CDS against subject CDS with scoring and threshold-based similarity.

  • Detailed log and Excel output of mapping results

  • Adjustable similarity thresholds and multi-processor usage.

Installation

pip install genemapper

Usage - Metadata Retrieval

genemapper metadata --query my_query_gene_cds.fasta --reference my_subject_gene_cds.fasta --email you@example.com

Usage - Gene Mapping

genemapper map --query my_query_gene_cds.fasta --subject my_subject_gene_cds.fasta --output LAbriniVersusCAETHG_Mapping.xlsx --similarityThreshold 0.99 --numProcesses 20

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