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A command-line tool for retrieving GenBank metadata and mapping coding sequences between query and subject FASTA files to identify their most similar counterparts.

Project description

PyPI version Build Status Documentation Status BioPython Pandas Python version

A command-line tool for retrieving GenBank metadata and mapping coding sequences between query and subject FASTA files to identify their most similar counterparts.

Features

  • Automated retrieval of GenBank metadata for query and reference sequences files in FASTA format.

  • Parallelized mapping of query CDS against subject CDS with scoring and threshold-based similarity.

  • Detailed log and Excel output of mapping results

  • Adjustable similarity thresholds and multi-processor usage.

Installation

pip install genemapper

Usage - Metadata Retrieval

genemapper metadata --query my_query_gene_cds.fasta --reference my_subject_gene_cds.fasta --email you@example.com

Usage - Gene Mapping

genemapper map --query my_query_gene_cds.fasta --subject my_subject_gene_cds.fasta --output LAbriniVersusCAETHG_Mapping.xlsx --similarityThreshold 0.99 --numProcesses 20

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