hs2p 4.0.3

Optimized slide tiling library for histopathology

hs2p

hs2p is a Python package for fast, scalable whole-slide tiling and annotation-aware sampling. You can request tiles at any spacing, whether or not that spacing is natively present in the image pyramid. It is designed for computational pathology workflows that need reproducible coordinates, explicit artifacts, and backend-independent physical semantics.

We support two main workflows:

• a Python API for library-style integration
• a CLI for batch preprocessing

Demo

Try hs2p interactively: hs2p-demo on HuggingFace Spaces
You can adjust tiling parameters and inspect the resulting grid and mask previews.
You can also upload your own pyramidal WSI (up to 1 GB).

Installation

Base install:

pip install hs2p

Optional backend extras:

pip install "hs2p[openslide]"
pip install "hs2p[asap]"
pip install "hs2p[vips]"
pip install "hs2p[cucim]"
pip install "hs2p[all]"

The supported backend set is:

• auto
• cucim
• vips
• openslide
• asap

auto prefers cucim -> vips -> openslide -> asap.

SAM2-based tissue segmentation requires a separate additional install:

pip install "hs2p[sam2]"
pip install "git+https://github.com/facebookresearch/sam2.git"

Workflows

Tiling

Tiling computes a reproducible grid of tile coordinates for each slide and saves them as explicit named artifacts. When a precomputed tissue mask is not provided, hs2p segments tissue on the fly. If you want to create those masks ahead of time, a standalone script is available.

Sampling

Sampling filters or partitions tile coordinates by annotation coverage so you can keep only tiles relevant to a label or tissue class.

Python API

Minimal tiling example:

from pathlib import Path

from hs2p import (
    SlideSpec,
    TilingConfig,
    tile_slide,
    save_tiling_result,
    write_tiling_preview,
)

result = tile_slide(
    SlideSpec(
        sample_id="slide-1",
        image_path=Path("/data/wsi/slide-1.tif"),
        mask_path=Path("/data/mask/slide-1-tissue-mask.tif"), # optional
    ),
    tiling=TilingConfig(
        backend="openslide",
        requested_spacing_um=0.5,
        requested_tile_size_px=224,
        tolerance=0.07,
        overlap=0.0,
        tissue_threshold=0.1,
    ),
)

# save tiling results to disk
artifacts = save_tiling_result(result, output_dir=Path("output"))

print(artifacts.coordinates_npz_path)   # output/tiles/slide-1.coordinates.npz
print(artifacts.coordinates_meta_path)  # output/tiles/slide-1.coordinates.meta.json

# preview tile grid
tiling_preview_path = write_tiling_preview(
    result=result,
    output_dir=Path("output"),
    downsample=32,
)
print(tiling_preview_path)  # output/preview/tiling/slide-1.jpg

result is a canonical hs2p.preprocessing.TilingResult. Downstream code should use its structured fields such as:

• x
• y
• tissue_fractions
• tile_index
• requested_*
• effective_*
• min_tissue_fraction

More API details: docs/api.md

CLI

The CLI is intended for fast batch processing of multiple slides with the same config.
Both entrypoints read the same public mask_path column, and the command determines whether that path is treated as a tissue mask or an annotation mask:

Tiling csv (mask_path is optional and means a tissue mask here):

sample_id,image_path,mask_path
slide-1,/data/wsi/slide-1.tif,/data/mask/slide-1-tissue-mask.tif
slide-2,/data/wsi/slide-2.tif,
...

Sampling csv (mask_path is mandatory and means an annotation mask here):

sample_id,image_path,mask_path
slide-1,/data/wsi/slide-1.tif,/data/mask/slide-1-annotations.tif
slide-2,/data/wsi/slide-2.tif,/data/mask/slide-2-annotations.tif
...

Run:

hs2p /path/to/config.yaml

For a first run, start from hs2p/configs/default.yaml and edit only the essentials:

• csv
• output_dir
• tiling.backend
• tiling.params.requested_spacing_um
• tiling.params.requested_tile_size_px

More details about CLI: docs/cli.md

Outputs

hs2p writes explicit named artifacts rather than anonymous coordinate dumps.

• Tiling writes tiles/{sample_id}.coordinates.npz and tiles/{sample_id}.coordinates.meta.json
• Sampling writes the same pair under tiles/<annotation>/
• Batch runs also write process_list.csv
• Saved coordinate arrays use a deterministic order: numeric x first, then numeric y within each shared x

Artifact field reference: docs/artifacts.md

Docker

If you prefer running hs2p in a container, a published Docker image is available:

docker pull waticlems/hs2p:latest
docker run --rm -it -v /path/to/your/data:/data waticlems/hs2p:latest

Documentation

• Documentation index
• Python API guide
• CLI guide
• Artifact format reference
• Benchmark notes
• Tissue mask generation script