Interface to the image correlation spectroscopy library ICSx64.dll
Project description
ICSdll is a Python/ctypes interface to the Image Correlation Spectroscopy Dynamic Link Library (ICSx64.dll) developed at the Laboratory for Fluorescence Dynamics (LFD) for the Globals for Images SimFCS software.
ICSx64.dll is implemented in C++ using the Intel(r) Math Kernel Library and OpenMP. It provides functions and classes for the analysis of fluorescence time series data:
- 1D, 2D, and 3D auto- and cross-correlation
- Image pair correlation function (ipCF)
- Airy detector pair correlation function (apCF)
- Image mean square displacement (iMSD)
- Line spatio-temporal image correlation spectroscopy (lSTICS)
- Fit 1D pair correlation functions to the results of ipCF analysis
- Subtract immobile fractions
- Correct photo-bleaching
| Author: | Christoph Gohlke |
|---|---|
| Organization: | Laboratory for Fluorescence Dynamics. University of California, Irvine |
| License: | 3-clause BSD |
| Version: | 2019.7.10 |
Requirements
Revisions
- 2019.7.10
- Pass 22 tests. Wrap apcf, imsd, and lstics functions. Raise IcsError in case of DLL function errors. Use ICSx64.DLL version 2019.7.10.
- 2019.5.22
- Initial release based on ICSx64.DLL version 2018.7.18.
Notes
ICSdll is currently developed, built, and tested on 64-bit Windows only.
The API is not stable and might change between revisions.
Refer to the C++ header file and source code for function signatures.
References
- ipcf.ipynb Pair correlation function analysis of fluorescence fluctuations in big image time series using Python.
- Globals for Images SimFCS, Software for fluorescence image acquisition, analysis, and simulation.
- Globals for Airyscan, Image correlation analysis for the ZEISS(tm) LSM 880 Airyscan detector.
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