illico

illico is a python library performing fast and lightweight wilcoxon rank-sum tests (same as scanpy.tl.rank_genes_groups(…, method="wilcoxon")), useful for single-cell RNASeq data analyses and processing. Approximate speed benchmarks (done on a 8-CPUs, 1 GPU machine) ran on k562-essential (~300k cells, 8k genes, 2k perturbations) can be found below.

Test	Format	illico	scanpy	pdex	rapids-singlecell (GPU)
OVO (reference="non-targeting")	Dense	~20s	~1h	~20min	~25min
OVO (reference="non-targeting")	Sparse	~15s	~1h30min	~8min	~1h10min
OVR (reference=None)	Dense	~10s	>10h	>10h	~1min
OVR (reference=None)	Sparse	~10s	>10h	>10h	~1min

Installation

illico is compatible with python 3.11 and onward:

pip install illico -U

A dask extra is available if you wish to use dask.

Getting started

The full documentation is available here. A simple starter could be:

import anndata as ad
from illico import asymptotic_wilcoxon

adata = ad.read_h5ad('dataset.h5ad') # (n_cells, n_genes)
de_genes = asymptotic_wilcoxon(
       adata,
       # layer="Y", # <-- If you want tests to run not on .X, but a specific layer
       group_keys="perturbation",
       reference=["non-targeting"|None], # <- `None` computes cluster-wise DE genes. Any other `str` will be interpreted as label of the control cells.
       is_log1p=[False|True], # <-- Specify if your data underwent log1p or not
       return_as_scanpy=[False|True], # <-- Whether to return a dict compatible with Scanpy's `rank_genes_groups` function, or a pd.DataFrame holding all p-values, statistics, and fold-change
       )
# Eventually, if return_as_scanpy=True:
adata.uns["rank_genes_groups"] = de_genes

Release notes

See the changelog.

Why illico

The name illico is a wordplay inspired by the R package presto (now the Wilcoxon rank-sum test backend in Seurat). Aside from this naming reference, there is no affiliation or intended equivalence between the two. illico was developed independently, and although the statistical methodology may be similar, it was not designed to reproduce presto’s results.

Other tools available

scanpy also implements OVO and OVR asymptotic wilcoxon rank-sum tests.
pdex also implements OVO and OVR wilcoxon rank-sum tests.
As of March 2026, rapids-singlecell also implements OVO and OVR asymptotic wilcoxon rank-sum tests on GPU, with a focus on out-of-core datasets. If you are working with large datasets that do not fit in memory, you should check it out. For in-memory datasets, illico was benchmarked to be faster, even if CPU based.

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BLAKE2b-256	`3f77f834e8dc2876bc9f1e885d24a4849540b9289783fb20c587246df5b44369`

Algorithm	Hash digest
SHA256	`913b146bbc05343a95484bd47d4bd7979bbeaa003fd639957c09e191e2f397cc`
MD5	`115233e0ab8cd8fce38d09edf3a19e80`
BLAKE2b-256	`35b4b814edd1c15be54f472cbf8425562960980a852af1fd023b403247ccfc58`

Algorithm	Hash digest
SHA256	`d9df8509179820d4a1951c9857ad913ecae933af34ecb6c3e1cc9f6b9dd608db`
MD5	`842561915557d53d419145bd1aaeee4a`
BLAKE2b-256	`0dfef5b583c37c96b6b5c3b8af9ccbecfad8854a6f7b8ec6b7b57bd2b44aec75`

Algorithm	Hash digest
SHA256	`d0b36ed248b848a67791c446449995f22aa615e10a2c6f3df7d20d15318dcf6f`
MD5	`0d6eecf0aafbfaa9b4d53fc2d4cd8240`
BLAKE2b-256	`d37d054e8825d7e84ca857f3460f99e2ed612e37631f4f349709be188d3c0a90`

Algorithm	Hash digest
SHA256	`0b17a23656b46795a62e98e50bc0d3c92a3b17c8fe62066609736ae2240b0988`
MD5	`e0c5d7cb52e81a493b05ed4bdcec4c20`
BLAKE2b-256	`06458602897e607e39fc5742ada026f2f5f42e46644357b32655fcc4913620c5`

Algorithm	Hash digest
SHA256	`a4a1a2c196c5368b797245422423407832e13335f5cdfc21156e0d2d0d3a53e3`
MD5	`292790cf544a0d9c4b642477162fbe8e`
BLAKE2b-256	`14bad68c334b5d912b4aa930493f79b0ad1623c171b62674abf11c3303679ec9`

Algorithm	Hash digest
SHA256	`0600a72744f897a599efd27208addcd2a4514a2402e02eb020f3a7d3a4058678`
MD5	`ff49d064db97b45883e4b8fd02e574f2`
BLAKE2b-256	`6c0010a11a04b51573f6653294a049eb2564309796a213272ce361974dac260c`

Algorithm	Hash digest
SHA256	`ee56f61b4f1841eb81691da40b10606854bb739941745c5d6af58a80a4d73718`
MD5	`6cd9d782c4f4f23f7ecdf20eea2a8f0f`
BLAKE2b-256	`9ecbb5d0068b3a146ae653da4ff1c7c0cb6d518b072a84fa9ec62b0a55dfb273`

Algorithm	Hash digest
SHA256	`bea36f949b71f84dae24b1454f985a54434640afcce7a20e4e855a711962573c`
MD5	`8dd17fb64c6774394c754fd2ed5cea88`
BLAKE2b-256	`4b4fbb7d04c8055a8e8a83068fb704c9860237be6aca64bf4c7a84ae82ad1bbb`

Algorithm	Hash digest
SHA256	`8cb882b9d200ce9253d9f9363a4836aea8e97cecb6b6fe7aced7463afd165135`
MD5	`3266c50e7dcd25274ad3dfb5bfecaaa2`
BLAKE2b-256	`e096c910a14d844d18896a7f0e3534dba75466476982ec85ff1ff31781930a3a`

Algorithm	Hash digest
SHA256	`ee72ac053d6b85b84d57c03d9fe0572216af198b926c596c05f16fe517d845aa`
MD5	`cf170537c24977a1472d9cec45dec9ad`
BLAKE2b-256	`1a8b0b236f8b2e68043fdf3da34c8e88d043c2701e0df57aeb2309e504704ea8`

Algorithm	Hash digest
SHA256	`71c15cb0842e88d7828e86f4b8c601891330f20b0554a629c3aa4e03f8cee368`
MD5	`cf2f867dd81980102a3b1081cfe424ea`
BLAKE2b-256	`0530402bd0d843e1fd6be1c071bbb4eb82a0e26242811d4ef71fecd8bde62907`

illico 0.6.0

illico

Installation

Getting started

Release notes

Why illico

Other tools available

Key dates

1 maintainer

Credits

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Provides Extra

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Source Distribution

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Algorithm	Hash digest
SHA256	`8a29589cd931e71890ae7c525bcc7c8ba16b7223ce426b767309869a30313e26`
MD5	`dbcb6315b1ffd569d5aa6df0ce058a5e`
BLAKE2b-256	`24b671eff4a84396592e5683908cac678aa0ef20de3835967abddf5876669c7d`

Algorithm	Hash digest
SHA256	`036071a3b9e4e9beb035bb0775eaa4efb7656576fcd533519215b06adc5453ac`
MD5	`10b2109ea6d8c96c0e22d49c76ea75c0`
BLAKE2b-256	`cf833db20aa52acd2dc4e31f7dd3e888d8707e6c495d3d8bf39869b1d2bd6c17`

Algorithm	Hash digest
SHA256	`58488fb0c21b2bc969f4aea850eb9f3c875fe6f87d4f52ec058190301594521a`
MD5	`396b6d5ba3b750b489d16fe3efd62466`
BLAKE2b-256	`9fcc8c2b0df48c2e5a0aa3e59319e250cc6ae313049f92588f6f4702866a11ac`

Algorithm	Hash digest
SHA256	`e8751a8f31fd89a39d8a4f09ef7dfac2312611b8d9ef0c3b956c6880c0c835ff`
MD5	`fc4242efc343812b13493ca967028dd3`
BLAKE2b-256	`7ab37b2f6ff2ecf311c422f441fc94eee4cfa8ec8c0ef87b1e83fdbc88ae3ab3`

Algorithm	Hash digest
SHA256	`8b20b3795c39554ccc5f5a649b373dd79a2d427b99a0d5e5089750801285640f`
MD5	`f7bf10de0e55af4f400b662d56c5495c`
BLAKE2b-256	`553348d770af3842fae4f7ca690b33702cc6118f1c423f503ebdec97e97e7c62`

Algorithm	Hash digest
SHA256	`ee34c237945b43dc498f7b06fea17813b25e3448c50b756a0289ccef4c71cba2`
MD5	`8647c05360c789af30376f09693024ac`
BLAKE2b-256	`fe87665b1e557f79472c0b2857ba956be898a3d56c1277de5746f7289291da37`

Algorithm	Hash digest
SHA256	`0f4d65d8a3a9c38551a1b0bce5ce1df9539fc4b2ccb2a0695f469a627aeb18b3`
MD5	`54376a0654d3eebfa4cdf29c08ec8d4c`
BLAKE2b-256	`eda584c0227d00509d9b2479895b3db4dd300a6220786ad8fb715abe86573c40`

Algorithm	Hash digest
SHA256	`7019e9b56aeb9f7cdb253935d0a7a3cbbc38687b515fc2f5f50f621348f0ae63`
MD5	`7cd3f3f3e900f53816eb439fd0496dae`
BLAKE2b-256	`2876eb7fedbd0573dba8aae3ccd2af28bb152b7b232ebed1725f95d6266021fb`

Algorithm	Hash digest
SHA256	`9fbb77e3a5643fcc9bc5052cc3f361722e4be3f0189620449a3b6ca47614952a`
MD5	`1e59e3e00d230d0ebfdb882e155a8189`
BLAKE2b-256	`db78c6bf025730c98b39a17fd58a4d1e4d5bd32611928058c79dece0d2dee56b`

Algorithm	Hash digest
SHA256	`0019552ed984ac5c1b648f47259c2b5c9566ade9d3adde22836ecd1902ff8899`
MD5	`1a7b7b8508b9fcd2acaeeccdcc9c3fb2`
BLAKE2b-256	`c24b4a3e80759741653d43327c162de0079a124fa944261116adeae6df2940ad`