Simple in silico PCR
Project description
ispcr
Documentation: https://pommevilla.github.io/ispcr
Source Code: https://github.com/pommevilla/ispcr
PyPI: https://pypi.org/project/ispcr/
A simple, light-weight package written in base Python to perform in silico PCR to determine primer performance.
Currently in development
Installation
pip install ispcr
Demonstration
The main function to use in this package is find_pcr_product
, which takes as input two file paths:
primer_file
- the path to fasta file containing your primers- This is currently limited to a fasta file containing two sequences, with the forward primer coming first and the reverse primer coming second
sequence_file
the path to the fasta file containing the sequences to test your primers against
find_pcr_product
will then iterate through the sequences in sequence
file and find all products amplified by the forward and reverse primer.
find_pcr_product
also takes a minimum_product_length
argument:
Project details
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