jacquard 0.42

Command-line tools to expedite analysis of Variant Call Format (VCF) files.

Suite of command-line tools to expedite analysis of exome variant data from multiple patients and multiple variant callers.

The official repository is at:

https://github.com/umich-brcf-bioinf/Jacquard

Usage

$ jacquard <subcommand> [options] [arguments]

Subcommands

translate:

Creates new VCFs, adding a controlled vocabulary of new FORMAT tags.

merge:

Integrates a directory of VCFs into a single VCF.

summarize:

Adds new INFO fields and FORMAT tags that combine variant data from the merged VCF.

expand:

Explodes a VCF file into a tab-delimited file.

For help on a specific subcommand:

$ jacquard <subcommand> --help

See ReadTheDocs for full documentation.

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Email [e-mail] for support and questions.

UM BRCF Bioinformatics Core

Installing Jaquard

Jacquard has been tested with Python 2.7 and 3.4 on Windows7, OSX, and *nix.

Prerequisites

Note

Pip installs all required libraries; see [Installing] below.

• natsort (3.5.2)

• nosetests, testfixtures (3.0.2), and numpy (>=1.7.1) are required for running automated tests

Installing

The easiest way to install Jacquard is through PyPI. Get pip if it’s not available in your system:

$ pip install jacquard

You can install from source from github:

$ pip install git+https://github.com/umich-brcf-bioinf/Jacquard

If you don’t have root permissions, you can install locally:

$ pip install --user jacquard

Note

You may need to modify your path to include the Python install dir (e.g. /Users/<username>/.local/bin)

Changelog

0.42 (X/X/XXXX)

• Added docs on readthedocs.

• Improved workflow documentation with example data

• Merge will now disambiguate tag collisions from multiple VCs

• Translate/summarize now supports GT tags

• Extended precision to 4 decimal places to support analysis of gene-panels.

• Adjusted translate to handle empty high-confidence VarScan files.

0.41 (5/7/2015)

• Combined filter command with merge command

• Extended expand to create simple metaheader glossary

• Adjusted code to support Python >=2.7 or 3.x

• Improved checks for consistent VCF file sets

• Fixed bug in merge that caused error if any VCFs were unsorted

• Fixed bug in summarize that caused error if variant was called by subset of callers

0.31 (3/17/2015)

• Downgraded VCF format from 4.2 to 4.1

• Fixed a bug that omitted CALLERS_REPORTED_LIST summary tag

• Simplified summary tags; removed dependency on numpy

• Adjusted VarScan translation to accept a file pattern to identify high-confidence files

0.3 (3/9/2015)

• Replaced normalize, tag commands with translate; relaxed constraints on incoming data.

• Renamed consensus to summarize

• More consistent behavior in expand

• Significantly improved merge performance

• Added new summary tags: - CALLERS_REPORTED_COUNT - CALLERS_REPORTED_LIST - SAMPLES_REPORTED_COUNT - CALLERS_PASSED_COUNT - CALLERS_PASSED_LIST - SAMPLES_PASSED_COUNT

• Fixed bug in how Strelka calculated AF on indels

• Improved command validation and error handling

• Added project/code documentation

• Removed dependencies on pandas

0.21 (10/2014)

• Initial public release

Jacquard is written and maintained by the University of Michigan BRCF Bioinformatic Core; individual contributors include:

• Jessica Bene

• Ashwini Bhasi

• Chris Gates

• Divya Kriti

• Kevin Meng

• Peter Ulintz