MAGIC
Project description
Markov Affinity-based Graph Imputation of Cells (MAGIC) is an algorithm for denoising and imputation of single cells applied to single-cell RNA sequencing data, as described in Van Dijk D et al. (2018), Recovering Gene Interactions from Single-Cell Data Using Data Diffusion, Cell https://www.cell.com/cell/abstract/S0092-8674(18)30724-4.
For R and MATLAB implementations of MAGIC, see https://github.com/KrishnaswamyLab/MAGIC.
Magic reveals the interaction between Vimentin (VIM), Cadherin-1 (CDH1), and Zinc finger E-box-binding homeobox 1 (ZEB1, encoded by colors).
Installation
Installation with pip
To install with pip, run the following from a terminal:
pip install --user magic-impute
Installation from GitHub
To clone the repository and install manually, run the following from a terminal:
git clone git://github.com/KrishnaswamyLab/MAGIC.git cd MAGIC/python python setup.py install --user
Usage
Example data
The following code runs MAGIC on test data located in the MAGIC repository:
import magic import pandas as pd import matplotlib.pyplot as plt X = pd.read_csv("MAGIC/data/test_data.csv") magic_operator = magic.MAGIC() X_magic = magic_operator.fit_transform(X, genes=['VIM', 'CDH1', 'ZEB1']) plt.scatter(X_magic['VIM'], X_magic['CDH1'], c=X_magic['ZEB1'], s=1, cmap='inferno') plt.show() magic.plot.animate_magic(X, gene_x='VIM', gene_y='CDH1', gene_color='ZEB1', operator=magic_operator)
Interactive command line
We have included two tutorial notebooks on MAGIC usage and results visualization for single cell RNA-seq data.
EMT data notebook: http://nbviewer.jupyter.org/github/KrishnaswamyLab/magic/blob/master/python/tutorial_notebooks/emt_tutorial.ipynb
Bone Marrow data notebook: http://nbviewer.jupyter.org/github/KrishnaswamyLab/magic/blob/master/python/tutorial_notebooks/bonemarrow_tutorial.ipynb
Help
If you have any questions or require assistance using MAGIC, please contact us at https://krishnaswamylab.org/get-help.
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