napari-flopa
Work in progress — the plugin is functional but under active development. Expect breaking changes between versions.
A napari plugin for opening, processing and analysing FLIM (Fluorescence Lifetime Imaging Microscopy) data from .ptu files.
Features
- Process PTU — reconstruct
.ptufiles into xarray datasets (photon count, mean arrival time, phasor, TCSPC histogram); supports multi-frame, multi-sequence and multi-detector data - FLIM View — interactive display with histogram contrast sliders for intensity and lifetime; FLIM RGB composite; export to TIFF/PNG
- Phasor — phasor plot with calibration, smoothing, per-object or per-pixel scatter, monoexponential lifetime semi-circle overlay
- Decay — TCSPC decay plot with aggregation, normalisation and log scale
- Batch — NOW DISABLED process a folder of
.ptufiles with a shared scan config and export images, phasor tables and decay tables; config saved/loaded as json
Installation
⚠️ Not yet released on PyPI. Install from source:
git clone https://github.com/cockovaz/napari-flopa
cd napari-flopa
pip install -e ".[all]"
License
Distributed under the terms of the MIT license.
napari-flopa is free and open source software.
Issues
If you encounter any problems, please file an issue along with a detailed description.
1 maintainer
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