o2-processing-utils

Tool for running and logging various bioinformatics tools on Harvard Medical School's high performance computing cluster O2.

Installation

Conda environment

To install the package, you can first install conda and then create a conda environment using the provided .yml file with the following command: conda env create -f o2_processing_utils.yml. The environment can then be activated with the command conda activate o2_processing_utils.

Manual installation

You can also set up the environment manually. Run pip install o2_processing_utils to install the package. You also need to install samtools, pbmm2 1.13.*, and at least Python 3.8.

Usage

After installation, first ensure that the environment variable O2_PROCESSING_CONFIG is set to the absolute path of the .json file containing the path to the reference file, the path to the log file, and the resources to be allocated to the Slurm job running pbmm2. You can set the environment variable using the command

export O2_PROCESSING_CONFIG=/PATH_TO_CONFIG/config.json

An example configuration file (config.example.json) is provided.

To analyze a PacBio HiFi/Fiber-Seq unaligned BAM, repeatedly run the following command from the command line:

o2p-run-pbmm2-workflow -b <input.bam>

Each time the command is run, a single step will be performed on the file or files of interest; as a result, you will have to run the same command several times on the same file. Repeat runs of the command will automatically perform the next analysis if the previous step was completed successfully. The workflow for a file is finished once you receive the message "The workflow is complete for file {file_name}. Nothing else is done for this file". The steps that are run include alignment, performing basic checks, gathering QC metrics, and parsing QC metrics. The tool will automatically submit Slurm jobs for the steps if needed. To analyze multiple samples simultaneously, the user can pass the folder path containing the unaligned BAM files as an argument to the command with the -f flag.

The following commands are provided for additional functionality:

For additional information, you can type any of the following commands into the command line followed by the flag --help. If you forget any of the available commands, you can also type o2p- into the command line and then hit TAB twice. This will display all of the available functions.

The currently supported alignment tools are:

• pbmm2 v1.13.* (employs minimap2 v2.26)

The currently supported QC tools are:

• samtools (samtools stats)

Development

To develop this package, clone this repo, make sure poetry is installed on your system and run make install.