phenocluster 0.1.0

Clinical Phenotype Discovery using Latent Class / Profile Analysis with Automatic Model Selection

A flexible data-driven framework for identifying clinical phenotypes using latent class and profile analysis

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Overview

PhenoCluster is a Python framework for unsupervised discovery of clinical phenotypes from heterogeneous patient data. It implements an end-to-end pipeline: from data preprocessing and latent class identification to outcome association analysis, survival modelling, and multistate transition modelling.

The framework is domain-agnostic and can be applied to any clinical cohort study where the goal is to identify latent patient subgroups and characterise their relationship with clinical outcomes. Users supply a dataset and a YAML configuration file; PhenoCluster handles model selection, phenotype assignment, and downstream inference automatically.

Key capabilities

• Latent Class / Profile Analysis via the StepMix framework with native support for mixed continuous/categorical data and missing values
• Automatic model selection using information criteria (BIC, AIC, ICL, CAIC, SABIC) with configurable cluster-size constraints
• Classification quality assessment with per-phenotype Average Posterior Probability (AvePP) and assignment confidence metrics
• Outcome association analysis with logistic regression yielding odds ratios, confidence intervals, and FDR-corrected p-values
• Survival analysis with Cox proportional hazards models producing hazard ratios and log-rank tests
• Multistate modelling with transition-specific Cox PH analysis, Monte Carlo simulation for state occupation probabilities with confidence interval bands, and clinical pathway enumeration
• Comprehensive output including an interactive HTML report, forest plots with confidence intervals, Kaplan-Meier and Nelson-Aalen curves, heatmaps, and JSON/CSV data exports

Installation

Requires Python ≥ 3.11

From PyPI

pip install phenocluster

From source

git clone https://github.com/EttoreRocchi/PhenoCluster.git
cd PhenoCluster
pip install -e ".[dev]"

Quick start

1. Generate a configuration file

phenocluster create-config -p complete -o config.yaml

2. Edit the configuration

Open config.yaml and fill in your dataset-specific parameters:

global:
  project_name: "My Study"
  output_dir: "results"
  random_state: 42

data:
  continuous_columns:
    - age
    - bmi
    - lab_value_1
  categorical_columns:
    - sex
    - smoking_status
    - disease_stage
  split:
    test_size: 0.2

outcome:
  enabled: true
  outcome_columns:
    - mortality_30d
    - readmission_30d

survival:
  enabled: true
  targets:
    - name: "overall_survival"
      time_column: "time_to_death"
      event_column: "death_indicator"

3. Run the pipeline

phenocluster run -d data.csv -c config.yaml

4. Inspect results

Results are written to the output directory (default: results/):

File	Description
analysis_report.html	Comprehensive HTML report with all results and visualisations
cluster_statistics.json	Phenotype sizes, feature distributions, and classification quality
outcome_results.json	Odds ratios with confidence intervals and p-values
survival_results.json	Kaplan-Meier estimates and Cox PH hazard ratios
multistate_results.json	Transition-specific hazard ratios, pathways, and state occupation
data/model_fit_metrics.csv	Information criteria, entropy, and average posterior probabilities
data/phenotypes_data.csv	Original data augmented with phenotype assignments
data/posterior_probabilities.csv	Posterior class membership probabilities
results/model_selection_summary.json	Model selection comparison table and best model info
results/feature_importance.json	Feature characterisation per phenotype
results/validation_report.json	Internal validation metrics (train/test comparison)
results/stability_results.json	Consensus clustering stability metrics
results/split_info.json	Train/test split details
results/external_validation_results.json	External validation results (when enabled)
phenocluster.log	Pipeline execution log
artifacts/	Cached intermediate results for incremental re-runs

Pipeline overview

PhenoCluster executes the following stages in order:

1. Data quality assessment. Missingness patterns, correlations, variance, and MCAR testing.
2. Train/test split. Stratified splitting with configurable test size, performed before preprocessing to prevent data leakage.
3. Preprocessing. Imputation, outlier handling, categorical encoding, standardization, and feature selection -- fit on training data only, then applied to the test set.
4. Model selection. Cross-validated information criterion search over cluster counts (training set only).
5. Full-cohort refit. Once K is selected, preprocessing and LCA/LPA model are refitted on the entire cohort; phenotypes reordered by size (largest = Phenotype 0).
6. Stability analysis. Consensus clustering over subsampled runs.
7. Internal validation. Train/test log-likelihood comparison, cluster proportion stability, and outcome OR consistency.
8. Outcome association. Logistic regression for binary outcomes with FDR-corrected p-values (optional).
9. Survival analysis. Kaplan-Meier curves, Nelson-Aalen estimators, log-rank tests, and Cox PH hazard ratios (optional).
10. Multistate modelling. Transition-specific Cox PH models, transition hazard ratios, and Monte Carlo simulation (optional).
11. Report generation. Interactive HTML report with all figures and tables.

CLI reference

Command	Description
phenocluster run -d DATA -c CONFIG [--force-rerun]	Run the full pipeline
phenocluster create-config [-p PROFILE] [-o OUTPUT]	Generate a config YAML from a profile template
phenocluster validate-config -c CONFIG [-d DATA]	Validate config structure; cross-check columns against data
phenocluster version	Show version, repository link, and documentation link

Configuration profiles

Profiles set sensible defaults for common use-cases. Generate one with phenocluster create-config -p <profile>:

Profile	Description	Inference	Stability	Multistate
descriptive	Phenotype discovery only, no statistical inference	off	on	off
complete	All analyses enabled (outcomes, survival, multistate)	on	on	on
quick	Fast iteration for development	on	off	off

Configuration reference

See the full Configuration Reference in the documentation.

Documentation

Full documentation (statistical methods, configuration reference, output descriptions) is available at ettorerocchi.github.io/PhenoCluster.

Testing

pip install -e ".[dev]"
pytest tests/ -v

License

This project is licensed under the MIT License.

Citation

If you use PhenoCluster in your research, please cite:

Acknowledgment

This project relies on StepMix, a Python package for pseudo-likelihood estimation of generalized mixture models with external variables. We thank the authors for making their work openly available.

If you use this framework, please cite also:

Morin, S., Legault, R., Laliberté, F., Bakk, Z., Giguère, C.-É., de la Sablonnière, R., & Lacourse, É. (2025). StepMix: A Python Package for Pseudo-Likelihood Estimation of Generalized Mixture Models with External Variables. Journal of Statistical Software, 113(8), 1-39. doi: 10.18637/jss.v113.i08