primer3-py: simple oligo analysis and primer design
Primer3-py is a Python-abstracted API for the popular Primer3 library. The intention is to provide a simple and reliable interface for automated oligo analysis and design.
Routine oligo analysis is simple:
>>> import primer3
>>> primer3.calc_tm('GTAAAACGACGGCCAGT')
54.695494691112515
>>> primer3.calc_hairpin('CCCCCATCCGATCAGGGGG')
ThermoResult(structure_found=True, tm=66.75, dg=-3176.92, dh=-36300.00, ds=-106.80)
... and fast (~1000X faster than traditional subprocess wrappers):
In [1]: import primer3
In [2]: import tests.wrapper
In [3]: %timeit primer3.calc_tm('GTAAAACGACGGCCAGT')
100000 loops, best of 3: 4.74 us per loop
In [4]: %timeit test.wrappers.calc_tm('GTAAAACGACGGCCAGT')
100000 loops, best of 3: 5.78 ms per loop
Primer3-py also includes bindings for the Primer3 primer design engine
if you'd prefer to use an established pipeline. The IO parameters mirror those
of the original Primer3.
Please note that while we provide bindings, we do not provide support for the Primer3 design engine. Please contact the Primer3 dev team with your questions: https://github.com/primer3-org/primer3
A copy of the Primer3 2.6.1 design parameters manual can be found at: primer3 v2.6.1 manual
Documentation
Release files for primer3-py 2.3.1
For a detailed explanation of source distributions (sdists) and built distributions (wheels), please see the package formats documentation.
Source distribution (sdist)
| File | Size | Uploaded | |
|---|---|---|---|
| primer3_py-2.3.1.tar.gz | 703.1 kB | Details |
Built distributions (wheels)
Total release size: 50.7 MB
Release files / primer3_py-2.3.1.tar.gz
| Download URL | primer3_py-2.3.1.tar.gz |
|---|---|
| Size | 703.1 kB |
| Tags | Source |
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Release files / primer3_py-2.3.1-cp314-cp314t-win_amd64.whl
| Download URL | primer3_py-2.3.1-cp314-cp314t-win_amd64.whl |
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| Size | 1.6 MB |
| Tags | CPython 3.14 CPython 3.14 free-threading Windows x86-64 |
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| Download URL | primer3_py-2.3.1-cp314-cp314t-macosx_10_15_x86_64.whl |
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Release files / primer3_py-2.3.1-cp38-cp38-macosx_10_14_x86_64.whl
| Download URL | primer3_py-2.3.1-cp38-cp38-macosx_10_14_x86_64.whl |
|---|---|
| Size | 1.4 MB |
| Tags | CPython 3.8 macOS 10.14+ x86-64 |
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SHA-256 checksum How to use checksums |
70a4bf18b4f9b10d87b46940e46426a681a94cd8528ea1554a742aa1da7a1453
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twine/6.1.0 CPython/3.13.7
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