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Project Description

Primer3-py is a Python-abstracted API for the popular Primer3 library. The intention is to provide a simple and reliable interface for automated oligo analysis and design.

Routine oligo analysis is simple:

>>> import primer3
>>> primer3.calcTm('GTAAAACGACGGCCAGT')
49.16808228911765
>>> primer3.calcHairpin('CCCCCATCCGATCAGGGGG')
ThermoResult(structure_found=True, tm=34.15, dg=337.09, dh=-36300.00,
             ds=-118.13, msg=)

… and fast (~1000X faster than traditional subprocess wrappers):

In [1]: import primer3

In [2]: %timeit primer3.calcTm('GTAAAACGACGGCCAGT')
100000 loops, best of 3: 4.74 µs per loop

In [3]: %timeit primer3.wrappers.calcTm('GTAAAACGACGGCCAGT')
100000 loops, best of 3: 5.78 ms per loop

Primer3-py also includes bindings for the Primer3 primer design engine if you’d prefer to use an established pipeline. The IO parameters mirror those of the original Primer3.

Please note that while we provide bindings, we do not provide support for the Primer3 design engine. Please contact the Primer3 dev team with your questions: http://primer3.sourceforge.net/

A copy of the Primer3 2.3.7 design parameters manual can be found at: http://git.io/vnqBx

For documentation of the bindings, see https://libnano.github.io/primer3-py

Release History

Release History

0.5.1

This version

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0.5.0

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0.4.3

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0.4.2

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0.4.1

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0.4.0

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0.3.1

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0.3.0

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0.2.5

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0.2.4

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0.2.3

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0.2.2

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0.2.1

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0.2

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0.1

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Download Files

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File Name & Checksum SHA256 Checksum Help Version File Type Upload Date
primer3_py-0.5.1-cp27-cp27m-macosx_10_5_x86_64.whl (514.0 kB) Copy SHA256 Checksum SHA256 cp27 Wheel May 25, 2016
primer3_py-0.5.1-cp35-cp35m-macosx_10_5_x86_64.whl (517.3 kB) Copy SHA256 Checksum SHA256 cp35 Wheel May 25, 2016
primer3-py-0.5.1.macosx-10.5-x86_64.tar.gz (505.7 kB) Copy SHA256 Checksum SHA256 Source May 25, 2016
primer3-py-0.5.1.tar.gz (393.1 kB) Copy SHA256 Checksum SHA256 Source May 25, 2016

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