primer3-py

Simple primer design and analysis

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Project description

primer3-py: simple oligo analysis and primer design

Primer3-py is a Python-abstracted API for the popular Primer3 library. The intention is to provide a simple and reliable interface for automated oligo analysis and design.

Routine oligo analysis is simple:

>>> import primer3
>>> primer3.calc_tm('GTAAAACGACGGCCAGT')
49.16808228911765
>>> primer3.calc_hairpin('CCCCCATCCGATCAGGGGG')
ThermoResult(structure_found=True, tm=34.15, dg=337.09, dh=-36300.00, ds=-118.13)

... and fast (~1000X faster than traditional subprocess wrappers):

In [1]: import primer3

In [2]: import tests.wrapper

In [3]: %timeit primer3.calc_tm('GTAAAACGACGGCCAGT')
100000 loops, best of 3: 4.74 us per loop

In [4]: %timeit test.wrappers.calc_tm('GTAAAACGACGGCCAGT')
100000 loops, best of 3: 5.78 ms per loop

Primer3-py also includes bindings for the Primer3 primer design engine if you'd prefer to use an established pipeline. The IO parameters mirror those of the original Primer3.

Please note that while we provide bindings, we do not provide support for the Primer3 design engine. Please contact the Primer3 dev team with your questions: https://github.com/primer3-org/primer3

A copy of the Primer3 2.6.1 design parameters manual can be found at: primer3 v2.6.1 manual

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This version

2.3.0

Jan 6, 2026

2.2.0

May 20, 2025

2.1.0

Feb 26, 2025

2.0.3

Feb 16, 2024

2.0.2

Feb 9, 2024

2.0.1

Sep 19, 2023

2.0.0

May 31, 2023

1.2.2

May 16, 2023

1.2.1

Apr 28, 2023

1.2.0

Mar 22, 2023

1.1.0

Mar 1, 2023

1.0.0

Feb 11, 2023

0.6.1

May 10, 2020

0.6.0

May 12, 2019

0.5.8

May 12, 2019

0.5.7

Aug 10, 2018

0.5.6

Jul 13, 2018

0.5.5

Oct 30, 2017

0.5.4

Jun 21, 2017

0.5.3

May 6, 2017

0.5.2

May 2, 2017

0.5.1

May 25, 2016

0.5.0

Feb 26, 2016

0.4.3

Dec 1, 2015

0.4.2

Sep 18, 2015

0.4.1

Nov 28, 2014

0.4.0

Nov 26, 2014

0.3.1

Aug 14, 2014

0.3.0

Aug 13, 2014

0.2.5

Aug 7, 2014

0.2.4

Jun 18, 2014

0.2.3

Jun 6, 2014

0.2.2

Jun 4, 2014

0.2.1

Apr 25, 2014

0.2

Apr 24, 2014

0.1

Mar 21, 2014

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primer3-py 2.3.0

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primer3-py: simple oligo analysis and primer design

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