scipion-em-aretomo 4.0.0

Plugin to use aretomo within the Scipion framework

This plugin provides a wrapper for AreTomo program.

Installation

You will need to use 3.0+ version of Scipion to be able to run these protocols. To install the plugin, you have two options:

1. Stable version

scipion installp -p scipion-em-aretomo

2. Developer’s version

   • download repository

   git clone -b devel https://github.com/scipion-em/scipion-em-aretomo.git

   • install

   scipion installp -p /path/to/scipion-em-aretomo --devel

• AreTomo binaries will be installed automatically with the plugin, but you can also link an existing installation.

• Default installation path assumed is software/em/aretomo2-1.1.3, if you want to change it, set ARETOMO_HOME in scipion.conf file to the folder where the AreTomo is installed.

• If you need to use CUDA different from the one used during Scipion installation (defined by CUDA_LIB), you can add ARETOMO_CUDA_LIB variable to the config file. Various binaries can be downloaded from the official website.

To check the installation, simply run the following Scipion test:

scipion test aretomo.tests.test_protocols_aretomo

Licensing

AreTomo2 binaries are available under BSD-3 license

Supported versions

aretomo2: 1.1.3

Protocols

• tilt-series align and reconstruct

References

• AreTomo: An integrated software package for automated marker-free, motion-corrected cryo-electron tomographic alignment and reconstruction. Shawn Zheng, Georg Wolff, Garrett Greenan, Zhen Chen, Frank G. A. Faas, Montserrat Bárcena, Abraham J. Koster, Yifan Cheng, David Agard. JSB vol. 6, 2022, 100068.