reverse complement a set of FastQ files
This is is the revcomp pipeline from the Sequana projet
|Overview:||Simply reverse complement a bunch of fastq files|
|Output:||their reverse complement counterpart|
|Citation:||Cokelaer et al, (2017), ‘Sequana’: a Set of Snakemake NGS pipelines, Journal of Open Source Software, 2(16), 352, JOSS DOI doi:10.21105/joss.00352|
You must install Sequana first:
pip install sequana
Then, just install this package:
pip install sequana_revcomp
sequana_pipelines_revcomp --input-directory DATAPATH
This creates a directory with the pipeline and configuration file. You will then need to execute the pipeline:
cd revcomp sh revcomp.sh # for a local run make clean
This launch a snakemake pipeline. If you are familiar with snakemake, you can retrieve the pipeline itself and its configuration files and then execute the pipeline yourself with specific parameters:
snakemake -s revcomp.rules -c config.yaml --cores 4 --stats stats.txt
Or use sequanix interface.
This pipelines requires the following executable(s):
This pipeline runs seqtk in parallel on the input fastq files.
Rules and configuration details
Here is the latest documented configuration file to be used with the pipeline. Each rule used in the pipeline may have a section in the configuration file.
|0.8.2||Fix schema and rule. output files are now saved in the ./rc directory|
|0.8.1||Improve the –help message|
|0.8.0||First version from sequana 0.8.0|
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