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One-step genotyping tools for Nanopore amplicon sequencing

Project description

# DAJIN2 is now under development👷

I aim to release a stable version of DAJIN2 by March 2023:crossed_fingers: ## New planned features

### Wet experiments

  • [ ] support nCATs

  • [ ] support PacBio

### Functions

  • [ ] output VCF

  • [ ] multi-allele phasing

  • [x] trim microhomology in BAM

### Usability

  • [ ] easy installation - [ ] PyPI - [ ] BioConda - [ ] docker image

  • [ ] support low-/middle-spec laptop

  • [x] support macOS

  • [x] support samples without a reference genome

  • [x] support visualization using igv.js via DAJIN2 view command

  • [x] support graphical user interface using flask via DAJIN2 gui command

### Maintainability

  • [ ] tests

  • [x] minimize dependencies - [x] Python, R, Bash -> Python - [x] samtools -> pysam - [x] minimap2 -> mappy - [x] remove NanoSim - [x] remove Tensorflow (GPU computation)

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