Skip to main content

brainglobe-stitch

Stitching tiled 3D light-sheet data in napari

Stitching a mouse brain acquired at a resolution of 4.06 µm/px, 4.06 µm/px, 5 µm/px using 4 tiles


A napari plugin for stitching tiled 3D acquisitions from a mesoSPIM light-sheet microscope. The plugin utilises BigStitcher to align the tiles and napari to visualise the stitched data.

Installation

We strongly recommend to use a virtual environment manager (like conda). The installation instructions below will not specify the Qt backend for napari, and you will therefore need to install that separately. Please see the napari installation instructions for further advice on this.

To install latest development version:

pip install git+https://github.com/brainglobe/brainglobe-stitch.git

This plugin requires Fiji to be installed on your system. You can download Fiji here.

The BigStitcher plugin must be installed in Fiji. Please follow the instructions here.

Seeking help or contributing

We are always happy to help users of our tools, and welcome any contributions. If you would like to get in contact with us for any reason, please see the contact page of our website.

Citation

If you find this package useful, please make sure to cite the original BigStitcher publication:

Hörl, D., Rojas Rusak, F., Preusser, F. et al. BigStitcher: reconstructing high-resolution image datasets of cleared and expanded samples. Nat Methods 16, 870–874 (2019). https://doi.org/10.1038/s41592-019-0501-0

License

Distributed under the terms of the BSD-3 license, "brainglobe-stitch" is free and open source software

Acknowledgements

This napari plugin was generated with Cookiecutter using napari's cookiecutter-napari-plugin template and the Neuroinformatics Unit's template.

Metadata

Release files for brainglobe-stitch 0.0.1

For a detailed explanation of source distributions (sdists) and built distributions (wheels), please see the package formats documentation.

Source distribution (sdist)

Source distribution for brainglobe-stitch 0.0.1
File Size Uploaded
brainglobe_stitch-0.0.1.tar.gz 18.4 kB Details

Built distribution (wheel)

Table of built distributions (wheels) for brainglobe-stitch 0.0.1
File Interpreter ABI Platform
brainglobe_stitch-0.0.1-py3-none-any.whl Python 3 none any Details

Total release size: 35.7 kB

Release files / brainglobe_stitch-0.0.1.tar.gz

Download URL brainglobe_stitch-0.0.1.tar.gz
Size 18.4 kB
Tags Source
SHA-256 checksum
How to use checksums
3de11f9520ac5add2534cbaafc3e8945106f082cc2402986867b8cba95e725a9
BLAKE2b-256 checksum
How to use checksums
73159a80107c06f5e4ff835acfac9b9f03bb46027036ef847bf9c248337ca8e0
Upload date
Uploaded using Trusted Publishing?
What is trusted publishing?
No
Uploaded via twine/5.1.1 CPython/3.11.8

Release files / brainglobe_stitch-0.0.1-py3-none-any.whl

Download URL brainglobe_stitch-0.0.1-py3-none-any.whl
Size 17.4 kB
Tags Python 3
SHA-256 checksum
How to use checksums
571a10ed3a2e72e04cf240c3bf01060dac57bcc23891022dfb55e07ac8caaa01
BLAKE2b-256 checksum
How to use checksums
7e91884073a213bdd229ea8631a6a0bc7eeb83bba443d3e7d583e8a3d298696d
Upload date
Uploaded using Trusted Publishing?
What is trusted publishing?
No
Uploaded via twine/5.1.1 CPython/3.11.8
Anthropic, PBC Visionary sponsor Bloomberg Visionary sponsor Hudson River Trading Visionary sponsor Meta Visionary sponsor NVIDIA Visionary sponsor Microsoft Sustainability sponsor Depot Continuous Integration AWS Cloud computing and Security Sponsor Datadog Monitoring Fastly CDN Google Download Analytics Sentry Error logging StatusPage Status page