Chromatogram File Utils
Project description
Chromatogram File Utils
For Sanger sequencing data visualizing, alignment, mutation calling, and trimming etc.
Demo
command to generate the demo above
cfutils mut --query ./data/B5-M13R_B07.ab1 --subject ./data/ref.fa --outdir ./data/ --plot
How to install?
form pypi
(use this way ONLY, if you don't know what's going on)
pip install --user cfutils
manipulate the source code
- clone from github
git clone git@github.com:y9c/cfutils.git
- install the dependence
make init
- do unittest
make test
How to use?
- in the command line
cfutils mut --help
- or as a python module
import cfutils as cf
ChangeLog
- build as python package for pypi
- fix bug that highlighting wrong base
- replace blastn with buildin python aligner
TODO
-
call mutation by alignment and plot Chromatogram graphic
-
add a doc
-
change xaxis by peak location
-
fix bug that chromatogram switch pos after trim
-
wrap as a cli app
-
return quality score in output
-
fix issue that selected base is not in the middle
-
fix plot_chromatograph rendering bug
-
add projection feature to make align and assemble possible
Project details
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