fishfeats 1.2.1

Napari plugin for RNA-Fish+cells analysis pipeline

Fish&Feats

Napari plugin to quantify 3D cells in a tissue and their smRNA-Fish or other RNA contents.

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FishFeats offers several flexible options to analyse 3D cells and RNA counts, from segmentation of apical cells and nuclei to hierarchical clustering of cells based on their RNA contents. Installation/Usage are all described in the documentation.

Installation

Please refer to our documentation page for more details on the installation.

FishFeats is distributed as a pip module on pypi. It can be installed by typing in a python virtual environement:

pip install fishfeats

Some options of Fishfeats rely on dependencies that are not required by default, so to not force a lot of dependencies installation. If you want to do directly the installation with all dependencies, type:

pip install `fishfeats[full]`

Usage

You can launch fishfeats in Napari by going to Plugins>fishfeats>Start. It will open a file dialog box asking you to select the image that you want to analyze. Refer to the documentation for presentation of the different steps possible in the pipeline.

Test dataset

You can find in this zenodo repository freely available images that can be used to test our pipeline. All the steps are fully documented in the online documentation and can be performed with one of these test images.

Example of analysis you can do with FishFeats are detailled step-by-step here and can be followed with the test image.

License

Fishfeats is distributed freely under the BSD-3 license.