graftm 0.9.2

GraftM is a pipeline used for identifying and classifying marker gene reads from metagenomic datasets

# GraftM GraftM is a tool for finding genes of interest in metagenomes, metatranscriptomes, and whole genomes.

Using modular gene packages, GraftM will search the provided sequences using hmmsearch (HMMER) and place the identified sequences into a pre-constructed phylogenetic tree. The provides fast, phylogenetically informed community profiles and genome annotations. GraftM provides tools to: * Create and update custom gene packages to use with GraftM * Decorate trees, and of course * Analyse sequence datasets using these GraftM packages Head over to the [GraftM page](http://geronimp.github.io/graftM/) for more general information.

### Installation GraftM can be installed via pip: ` pip install graftm ` However, to use all features of GraftM a few extra binary applications are required: * orfm v. >= 0.2.0 (https://github.com/wwood/OrfM) * hmmer v. >= 3.1b1 (http://hmmer.janelia.org/) * fxtract (https://github.com/ctSkennerton/fxtract) * pplacer v. >= 2.6.32 (http://matsen.fhcrc.org/pplacer/) * krona v. >= 2.4 (http://sourceforge.net/p/krona/home/krona/) * mafft v. >= 7.22 (http://mafft.cbrc.jp/) * diamond v. >= 0.7.9 (https://github.com/bbuchfink/diamond)

To create new GraftM packages, you’ll also need * FastTreeMP (http://www.microbesonline.org/fasttree/)

### Manual A [manual](https://github.com/geronimp/graftM/wiki) is available in the form of the wiki here on GitHub.

### Contact If you have any further comments, complaints or recomendations about GraftM, drop an email to the [SupportM](https://groups.google.com/forum/?hl=en#!forum/supportm) public help forum. Software by [Joel A. Boyd](http://ecogenomic.org/users/joel-boyd) (@geronimp) and [Ben J. Woodcroft](http://www.ecogenomic.org/users/ben-woodcroft) (@wwood) at the [Australian Centre for Ecogenomics](http://ecogenomic.org) Released under GPL3 - see LICENCE.txt for licencing details