lfdfiles 2026.1.14

Laboratory for Fluorescence Dynamics (LFD) file formats

Lfdfiles is a Python library and console script for reading, writing, converting, and viewing many of the proprietary file formats used to store experimental data and metadata at the Laboratory for Fluorescence Dynamics. For example:

• SimFCS VPL, VPP, JRN, BIN, INT, CYL, REF, BH, BHZ, B64, I64, Z64, R64

• FLIMbox FBD, FBF, FBS.XML

• GLOBALS LIF, ASCII

• CCP4 MAP

• Vaa3D RAW

• Bio-Rad(r) PIC

• ISS Vista IFLI, IFI

• FlimFast FLIF

Author:

Christoph Gohlke

License:

BSD-3-Clause

Version:

2026.1.14

DOI:

10.5281/zenodo.8384166

Quickstart

Install the lfdfiles package and all dependencies from the Python Package Index:

python -m pip install -U "lfdfiles[all]"

Print the console script usage:

python -m lfdfiles --help

The lfdfiles library is type annotated and documented via docstrings.

See Examples for using the programming interface.

Source code and support are available on GitHub.

Requirements

This revision was tested with the following requirements and dependencies (other versions may work):

• CPython 3.11.9, 3.12.10, 3.13.11, 3.14.2 64-bit

• NumPy 2.4.1

• Tifffile 2026.1.14 (optional)

• Fbdfile 2026.1.14 (optional)

• Czifile 2019.7.2.1 (optional)

• Oiffile 2026.1.8 (optional)

• Netpbmfile 2026.1.8 (optional)

• Matplotlib 3.10.8 (optional, for plotting)

• Click 8.3.1 (optional, for command line apps)

Revisions

2026.1.14

• Improve code quality.

2025.12.12

• Derive LfdFileError from ValueError.

• Improve code quality.

2025.9.17

• Many breaking changes to FLIMbox functionality (use fbdfile package instead):

• Discourage use of FlimboxFbd, FlimboxFbf, and FlimboxFbs classes.

• Use fbdfile package to implement FlimboxFbd, FlimboxFbf, and FlimboxFbs.

• Remove flimbox_histogram, flimbox_decode, and sflim_decode functions.

• Remove convert_fbd2b64 function and fbd2b64 command line app.

• Remove deprecated SimfcsFbf, and SimfcsFbd classes.

• Remove deprecated simfcsfbd_histogram and simfcsfbd_decode functions.

2025.7.31

• Read variants of SimFCS REF files.

• Drop support for Python 3.10.

2025.5.10

• Mark Cython extension free-threading compatible.

• Remove doctest command line option.

• Support Python 3.14.

2025.3.16

• Replace deprecated tifffile.stripnull function.

• Fix misspelled VistaIfli.header keys.

• Drop support for Python 3.9.

2024.10.24

• …

Refer to the CHANGES file for older revisions.

Notes

The API is not stable yet and might change between revisions.

Python <= 3.10 is no longer supported. 32-bit versions are deprecated.

Many of the LFD file formats are not documented and might change arbitrarily. This implementation is mostly based on reverse engineering existing files. No guarantee can be made as to the correctness of code and documentation.

Experimental data are often stored in plain binary files with metadata available in separate, human readable journal files (.jrn).

Unless specified otherwise, data are stored in little-endian, C contiguous order.

The Laboratory for Fluorescence Dynamics (LFD) was a national research resource center for biomedical fluorescence spectroscopy funded by the National Institutes of Health from 1986 to 2022 (grant P41GM103540).

References

The following software is referenced in this module:

1. SimFCS, a.k.a. Globals for Images, is software for fluorescence image acquisition, analysis, and simulation, developed by Enrico Gratton at UCI.

2. Globals, a.k.a. Globals for Spectroscopy, is software for the analysis of multiple files from fluorescence spectroscopy, developed by Enrico Gratton at UIUC and UCI.

3. ImObj is software for image analysis, developed by LFD at UIUC. Implemented on Win16.

4. FlimFast is software for frequency-domain, full-field, fluorescence lifetime imaging at video rate, developed by Christoph Gohlke at UIUC.

5. FLImage is software for frequency-domain, full-field, fluorescence lifetime imaging, developed by Christoph Gohlke at UIUC. Implemented in LabVIEW.

6. FLIez is software for frequency-domain, full-field, fluorescence lifetime imaging, developed by Glen Redford at UIUC.

7. Flie is software for frequency-domain, full-field, fluorescence lifetime imaging, developed by Peter Schneider at MPIBPC. Implemented on a Sun UltraSPARC.

8. FLOP97 is software for frequency-domain, cuvette, fluorescence lifetime measurements, developed by Christoph Gohlke at MPIBPC. Implemented in LabVIEW.

9. VistaVision is commercial software for instrument control, data acquisition and data processing by ISS Inc (Champaign, IL).

10. Vaa3D is software for multi-dimensional data visualization and analysis, developed by the Hanchuan Peng group at the Allen Institute.

11. Voxx is a volume rendering program for 3D microscopy, developed by Jeff Clendenon et al. at the Indiana University.

12. CCP4, the Collaborative Computational Project No. 4, is software for macromolecular X-Ray crystallography.

Examples

Create a Bio-Rad PIC file from a NumPy array:

>>> data = numpy.arange(1000000).reshape((100, 100, 100)).astype('u1')
>>> bioradpic_write('_biorad.pic', data)

Read the volume data from the PIC file as NumPy array, and access metadata:

>>> with BioradPic('_biorad.pic') as f:
...     f.shape
...     f.spacing
...     data = f.asarray()
...
(100, 100, 100)
(1.0, 1.0, 1.0)

Convert the PIC file to a compressed TIFF file:

>>> with BioradPic('_biorad.pic') as f:
...     f.totiff('_biorad.tif', compression='zlib')
...