splicecraft 0.4.5

Terminal-based circular plasmid map viewer, sequence editor, and Primer3/Golden Braid primer design workbench

SpliceCraft

A terminal-based circular plasmid map viewer, sequence editor, primer design workbench, and Golden Braid parts domesticator — rendered entirely in your shell. Fetch any GenBank record by accession, load local files, organize plasmids into named collections, design diagnostic / cloning / Golden Braid primers with Primer3, run SOE-PCR site-directed mutagenesis on any CDS, and edit sequences — without ever leaving the terminal.

Quick start

⚠️ Beta software. SpliceCraft is under active development and the API, UI, and on-disk file formats may change between releases. Your data files (plasmid_library.json, parts_bin.json, primers.json) are auto-backed up to *.bak on every save, but please keep your own off-disk copies of anything critical. Do not use SpliceCraft as your sole system of record for cloning work until it exits beta.

pipx install splicecraft
splicecraft              # empty canvas
splicecraft L09137       # fetch pUC19 from NCBI
splicecraft myplasmid.gb # open a local GenBank (.gb/.gbk) or SnapGene (.dna) file

That's it. pipx creates an isolated virtual environment for SpliceCraft and its dependencies, so it won't clash with system Python packages. If you don't have pipx yet: sudo apt install pipx on Debian/Ubuntu/WSL2, brew install pipx on macOS, or python -m pip install --user pipx elsewhere. User data lives in the platform-appropriate data directory (see the Installation section below).

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Features

Core visualization & editing

• Braille dot-matrix circular map — plasmids rendered as crisp Unicode braille rings with per-strand feature arcs, directional arrowheads, and proximity-placed labels
• Linear map view — toggle with v for a horizontal strip layout
• Dithered sequence panel — per-base DNA viewer with feature bars, restriction site overlays, and double-stranded display
• Inline amino-acid translation — every CDS shows its protein right above (forward) or below (reverse) its DNA bar, one letter per codon midpoint, in the feature's colour. Wrap-around CDSes translate correctly across the origin. Click an AA letter to highlight that codon's three bases on the strand — Ctrl+C copies the codon
• Per-strand restriction-cut visualization — clicking a sticky cutter (EcoRI, HindIII, …) tints the upstream bases blue and downstream bases red, with the staggered overhang shown as different colours on the two strands
• Live NCBI fetch — pull any GenBank record by accession number on demand
• Local file support — open .gb / .gbk (GenBank) or .dna (SnapGene native) files directly from disk
• Free rotation — spin the origin with [ / ] or arrow keys (when the map has focus)
• Restriction enzyme overlay — 200+ NEB enzymes including Type IIS (BsaI, BsmBI, BbsI, SapI) with visible recognition arcs + cut markers

Libraries (all persist to JSON)

• Plasmid collections — organize plasmids into named buckets (e.g. "yeast project", "E. coli toolkit"). Library panel toggles between a collection list and the active collection's plasmids; a default "Main Collection" is created on first run, with full add / remove / rename CRUD from the panel
• Plasmid library — auto-saves on import, mirrors live into the active collection, survives restarts, rename + handslip-protected delete; unsaved edits prompt Save / Discard / Cancel before navigating away
• Library fuzzy search — type into the search box at the top of the library panel to filter the visible table by subsequence match (case-insensitive, not necessarily contiguous). Empty submit restores the full list.
• Parts Bin — Golden Braid L0 parts catalog with user-domesticated parts including sequences and primer pairs
• Primer library — all designed primers with Tm, length, date, status (Designed / Ordered / Validated), multi-select for batch operations
• Feature library — reusable feature snippets with per-entry color and strand, centralized workbench for browse / edit / rename / recolor / delete

Primer design (Primer3)

• Detection primers — diagnostic PCR; Primer3 picks the ideal pair within a selected region, 450-550 bp product by default (configurable)
• Cloning primers — RE-site tails + GCGC padding; 30+ common enzymes or type a custom recognition sequence
• Golden Braid primers — Esp3I / BsmBI domestication for all L0 positions (Promoter, 5' UTR, CDS, CDS-NS, C-tag, Terminator). Splitting L0 (Esp3I) from L1 (BsaI) lets domesticated parts survive the L1 Golden Gate reaction without re-cutting.
• Generic primers — simple binding primers, no tails
• Primers can be added to the plasmid map as primer_bind features
• Scrollable TextArea for custom sequence input; highlighted text = target

Mutagenesis

• SOE-PCR site-directed mutagenesis — design 4-primer SOE sets for any W140F-style mutation. CDS source can be the loaded plasmid, a library entry, a domesticated part from the Parts Bin, or a free-form protein sequence (auto-harmonized to the active codon table). Edge cases (mutation within 60 nt of a CDS end) auto-fall back to a 2-primer modified-outer PCR.

Feature operations

• Feature sidebar — click a row to highlight on map; click the map to select the feature under the cursor
• Undo/redo — 50-deep snapshot stack for all sequence edits
• Delete protection — focus-aware Delete key; confirmation modal (default focus = No) for library entries
• Clipboard — OSC-52 copy works in Windows Terminal, iTerm2, modern WSL

Data safety

• Atomic saves — all JSON files written via tempfile + os.replace
• Automatic backups — every save writes *.json.bak before overwriting
• Corrupt-file recovery — missing files don't crash; corrupt files auto- restore from .bak with a warning notification on startup

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Installation

Requires Python 3.10+.

With pipx (recommended)

pipx install splicecraft

pipx installs SpliceCraft (and its Textual / Biopython / primer3-py / platformdirs deps) into an isolated virtual environment and places the splicecraft command on your PATH. This is the right approach on modern Debian, Ubuntu, Fedora, and WSL2, where pip install into the system Python is blocked by PEP 668.

If you don't already have pipx:

sudo apt install pipx           # Debian / Ubuntu / WSL2
brew install pipx               # macOS
python -m pip install --user pipx  # everywhere else
pipx ensurepath                 # one-time; adds ~/.local/bin to PATH

With pip inside a venv

If you prefer a plain pip workflow, use a virtual environment:

python3 -m venv ~/.venvs/splicecraft
~/.venvs/splicecraft/bin/pip install splicecraft
~/.venvs/splicecraft/bin/splicecraft

(Plain pip install splicecraft into system Python works on older distros and inside conda envs, but will be rejected by PEP 668 on any recent Debian-family system — use pipx or a venv instead.)

User data location

User data (library, parts bin, primers) lives in the platform-appropriate data directory:

Platform	Path
Linux	~/.local/share/splicecraft/
macOS	~/Library/Application Support/splicecraft/
Windows	%APPDATA%\splicecraft\

Override with SPLICECRAFT_DATA_DIR=/path/to/dir splicecraft.

From source

git clone https://github.com/Binomica-Labs/SpliceCraft.git
cd SpliceCraft
pip install -e .        # inside a venv, or pass --break-system-packages

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Usage

splicecraft              # empty canvas
splicecraft L09137       # fetch pUC19 from NCBI on launch
splicecraft myplasmid.gb # open a local GenBank (.gb/.gbk) or SnapGene (.dna) file
splicecraft --version    # print version
splicecraft --help       # quick usage hint

If running from a git clone (pip install -e .), the same commands work; you can also still run python3 splicecraft.py directly.

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Key Bindings

Main screen

Key	Description
[ / ]	Rotate map origin left / right (when map focused)
← / →	Same as [ / ] (when map focused)
↑	Reset origin to 0 (when map focused)
Shift+[/]	Rotate coarse (10× step)
Home	Reset origin to 0
, / .	Circular map aspect wider / taller
v	Toggle circular ↔ linear map
l	Toggle feature label connector lines
r	Toggle restriction-site overlay
f	Fetch a record from NCBI by accession
Ctrl+O	Open a .gb file from disk
Ctrl+Shift+A	Add current plasmid to the library
Ctrl+E	Enter sequence editor mode
Ctrl+S	Save edits to file
Ctrl+F	Add a new feature (from cursor or blank)
Ctrl+Shift+F	Capture selection / feature → Feature library
Enter	Highlight the feature enclosing the seq cursor
Delete	Context-aware delete (feature or library entry)
Ctrl+Z	Undo
Ctrl+Shift+Z	Redo
Ctrl+C	Copy selection (top strand 5'→3', or AA when CDS highlighted)
Alt+C	Copy selection (bottom strand, reverse-complement)
q	Quit

Primer Design screen

Key	Description
esc	Close primer screen
m	Mark / unmark primer under cursor (★)
M	Mark / unmark all primers
S	Cycle status: Designed → Ordered → Validated
Tab	Cycle focus between fields

Mouse

Action	Description
Click DNA row	Place cursor at that base
Click feature bar	Highlight the feature, set cursor at its 5' end
Click AA letter	Highlight that codon's three bases on the strand
Click restriction site	Highlight recognition span; tint upstream blue / downstream red per strand
Double-click	Select full feature span
Drag	Select a sequence range
Scroll wheel	Rotate map (when over map panel)
Click backbone	Clear all panel highlights

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Menus

Menu	Items
File	Open .gb file · Fetch from NCBI · Add to Library · Save · Export GenBank · Collections · Quit
Edit	Edit Sequence · Undo · Redo · Add Feature · Capture → feat-lib · Delete Feature
Enzymes	Show RE sites · Unique cutters · 6+/4+ bp sites · Connectors
Features	Opens the Feature Library workbench
Primers	Opens the full-screen Primer Design workbench
Mutagenize	Opens the SOE-PCR site-directed mutagenesis designer (4-source CDS picker)
Parts	Opens the Parts Bin (Golden Braid L0 parts catalog)
Constructor	Opens the Assembly Constructor for TU building

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Requirements

Package	Version	Purpose
Python	≥ 3.10	Runtime
Textual	≥ 8.2.3	TUI framework and rendering engine
Rich	≥ 14.0	Terminal rendering (Textual dependency)
Biopython	≥ 1.87	GenBank parsing and NCBI Entrez fetch
primer3-py	≥ 2.3.0	Primer design (Tm, thermodynamic screening)
platformdirs	≥ 4.2	Cross-platform user-data directory
pytest	≥ 9.0	Test suite (dev only)
pytest-asyncio	≥ 1.3	Async test support (dev only)

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Data files

All user data persists as human-readable JSON in the platform-appropriate user data directory:

File	Purpose
collections.json	Named collections of plasmids; source of truth
plasmid_library.json	Live mirror of the active collection's plasmids
parts_bin.json	User-domesticated Golden Braid parts
primers.json	Designed primer library
features.json	Reusable feature snippets
feature_colors.json	Per-type feature color overrides
codon_tables.json	Cached codon-usage tables fetched from Kazusa
cloning_grammars.json	User-defined cloning grammars (Golden Braid / MoClo)
settings.json	App preferences (active collection, active grammar, ...)
*.json.bak	Automatic backup — written before each save

A manual backup rotation happens on every save so accidental data loss is always recoverable via the .bak file.

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Tests

python3 -m pytest -n auto -q                  # full suite (1,009 tests, ~2 min on 8 cores)
python3 -m pytest tests/test_dna_sanity.py    # biology correctness only (< 2s)

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Codebase tour

SpliceCraft is a single-file Python app (splicecraft.py, ~17,900 lines) built on Textual + Biopython. The single-file layout is intentional — no import puzzles, and everything is greppable from one place.

Layout at a glance

Region	Contents
Top of file	imports, user data dir (platformdirs), rotating session-tagged logger, _safe_save_json / _safe_load_json (atomic writes + .bak recovery)
Persistence	Library + parts-bin + primers + features + feature-colors + codon-tables + cloning-grammars + settings + collections (collection-driven model with active-pointer in settings.json + library mirror sync)
Biology core	NEB enzyme catalog, IUPAC-aware _rc, _scan_restriction_sites (palindrome- and wrap-aware), sequence panel renderer, _translate_cds
I/O	fetch_genbank (NCBI Entrez), load_genbank (.gb / .gbk / .dna), GenBank/FASTA export
Rendering	_Canvas + _BrailleCanvas (sub-cell dot matrix), PlasmidMap, SequencePanel, FeatureSidebar, LibraryPanel (two-mode: collections list ↔ active-collection plasmids), MenuBar
Workbenches	PrimerDesignScreen (detection / cloning / Golden Braid / generic), MutagenizeModal (SOE-PCR; 4-source CDS picker), DomesticatorModal + ConstructorModal (Golden Braid L0), FeatureLibraryScreen
Registries	codon-usage tables, parts bin, primer library, feature library, cloning grammars, plasmid collections — all persisted as JSON with automatic .bak backups
Controller	PlasmidApp — keybindings, undo/redo (50-deep snapshot stack), @work threads for NCBI / Kazusa, collection-driven startup migration in compose()

Contributor docs

CLAUDE.md at the repo root is the agent + contributor handover document. It covers the 10 sacred invariants (biology correctness rules with test coverage), the error-handling convention, performance notes, and modular recipes for adding modals, workers, menus, or persisted libraries without tripping the invariants. Read it before touching the rendering layer, record pipeline, or primer design.

Running the suite

python3 -m pytest -n auto -q                  # full suite (~2 min)
python3 -m pytest tests/test_dna_sanity.py    # biology only (< 2 s)

All tests run offline against synthetic SeqRecords and monkeypatched data paths; an autouse fixture in tests/conftest.py guarantees no test can write to real user files.

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License

MIT