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Bisulfite Sequencing Processing Platform

Project description

BSBolt (BiSulfite Bolt)

A fast and safe read alignment platform for bisulfite sequencing data

BiSuflite Bolt (BSBolt); a fast and scalable bisulfite sequencing analysis platform. BSBolt is an integrated analysis platform that offers support for bisulfite sequencing read simulation, alignment, methylation calling, data aggregation, and data imputation. BSBolt has been validated to work with a wide array of bisulfite sequencing data, including whole genome bisulfite sequencing (WGBS), reduced representative bisulfite sequencing data (RRBS), and targeted methylation sequencing data.


BSBolt documentation can be found at


At installation BSBolt compiles two external dependencies Bowtie2 and ART Read Simulation Tools under the terms of the GPLv3 License. Installation failures are generally due to external C libraries not being installed or not being on path.

pip3 install BSBolt -v
  1. Python Dependencies
  2. External C Dependencies
    • libgsl
    • zlib
    • libbz2
    • liblzma
    • libcurl
    • libcrypto

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Files for BSBolt, version 0.1.1
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