bsbolt 1.4.0

Bisulfite Sequencing Processing Platform

BSBolt (BiSulfite Bolt)

A fast and safe bisulfite sequencing processing platform

BiSuflite Bolt (BSBolt); a fast and scalable bisulfite sequencing processing platform. BSBolt is an integrated analysis platform that offers support for bisulfite sequencing read simulation, alignment, methylation calling, data aggregation, and data imputation. BSBolt has been validated to work with a wide array of bisulfite sequencing data,including whole genome bisulfite sequencing (WGBS), reduced representative bisulfite sequencing data (RRBS), and targeted methylation sequencing data. BSBolt utilizes forked versions of BWA and WGSIM for read alignment and read simulation respectively. BSBolt is released under the MIT license.

Documentation

BSBolt documentation can be found at bsbolt.readthedocs.io.

Installation

PyPi Installation

The easiest installation method is installing pre-compiled binaries using PyPi. Binaries are provided for python >=3.6 on unix like systems (macOS >=10.15 and linux).

pip3 install BSBolt --user

Installing from Source

Dependencies

• zlib-devel >= 1.2.3-29
• GCC >= 8.3.1

# clone the repository
git clone https://github.com/NuttyLogic/BSBolt.git
cd BSBolt
# compile and install package
pip3 install .

Installing from Source on macOS

Dependencies

• autoconf
• homebrew
• xcode

Installation from source requires xcode command line utilities, homebrew macOS package manager, and autoconf are installed. Xcode through the mac App Store, running the xcode installation command listed below, or as part of the homebrew macOS package manager installation. The full installation process can be completed as outlined below.

# install xcode utilities
xcode-select --install
# install homebrew
/bin/bash -c "$(curl -fsSL https://raw.githubusercontent.com/Homebrew/install/master/install.sh)"
# install autoconf
brew install autoconf
# optionally install python
brew install python3.8
# install BSBolt
pip3 install BSBolt