Assemble transcript sequences fragments around variants
Isovar assembles protein subsequences around mutations from cancer RNA-Seq data. Since Isovar uses sequenced reads to determine a mutant coding sequence it is able to correctly phase somatic variants with adjacent germline variants, as well as sometimes recovering alternatively spliced isoforms.
$ isovar-protein-sequences \ --vcf somatic-variants.vcf \ --bam rnaseq.bam \ --min-reads 2 \ --protein-sequence-length 30 \ --output isovar-results.csv chr pos ref alt amino_acids \ 0 22 46931060 A C FGVEAVDHGWPSMSSGSSWRASRGPPPPPR variant_aa_interval_start variant_aa_interval_end ends_with_stop_codon \ 0 16 17 False frameshift translations_count supporting_variant_reads_count \ 0 False 1 1 total_variant_reads supporting_transcripts_count total_transcripts gene 0 130 2 2 CELSR1
The one line explanation of Isovar: ProteinSequence = VariantSequence + ReferenceContext.
A little more detail about the algorithm: 1. Scan through an RNAseq BAM file and extract sequences overlapping a variant locus (represented by LocusRead) 2. Make sure that the read contains the variant allele and split its sequence into prefix/alt/suffix string parts (represented by AlleleRead) 3. Assemble overlapping AlleleReads (which agree with the variant allele) into a VariantSequence 4. Gather possible reading frames for distinct reference sequences around the variant locus (represented by ReferenceContext). 5. Use the reading frame from a ReferenceContext to translate a VariantSequence into a protein fragment (represented by Translation). 6. Multiple distinct variant sequences and reference contexts can generate the same translations, so we aggregate those equivalent Translation objects into a ProteinSequence.
Since we may not want to deal with every possible translation of every distinct sequence detected around a variant, Isovar sorts the variant sequences by the number of supporting reads and the reference contexts in order of protein length and a configurable number of translated protein fragments can be kept from this ordering.
Isovar works best with high quality / high coverage mRNA sequence data. This means that you will get best results from >100M paired-end reads sequenced on an Illumina HiSeq from a library enriched with poly-A capture. The number of reads varies depending on degree of RNA degradation and tumor purity. The read length will determine the longest protein sequence you can recover, since Isovar’s cDNA assembly only considers reads that overlap a variant. With 100bp reads you will be able to assemble at most 199bp of sequence around a somatic single nucleotide variant, and consequently only be to determine 66 amino acids from the protein sequence. If you disable the cDNA assembly algorithm then a 100bp read will only be able to determine 33 amino acids.