🐍⛓️🧬 Pyskani 
PyO3 bindings and Python interface to skani, a method for fast fast genomic identity calculation using sparse chaining.
🗺️ Overview
skani[1] is a method developed by Jim Shaw
and Yun William Yu for fast and robust
metagenomic sequence comparison through sparse chaining. It improves on
FastANI by being more accurate and much faster, while requiring less memory.
pyskani is a Python module, implemented using the PyO3
framework, that provides bindings to skani. It directly links to the
skani code, which has the following advantages over CLI wrappers:
- pre-built wheels:
pyskaniis distributed on PyPI and features pre-built wheels for common platforms, including x86-64 and Arm64 UNIX. - single dependency: If your software or your analysis pipeline is
distributed as a Python package, you can add
pyskanias a dependency to your project, and stop worrying about theskanibinary being present on the end-user machine. - sans I/O: Everything happens in memory, in Python objects you control,
making it easier to pass your sequences to
skaniwithout having to write them to a temporary file.
This library is still a work-in-progress, and in an experimental stage, but it should already pack enough features to be used in a standard pipeline.
🔧 Installing
Pyskani can be installed directly from PyPI, which hosts some pre-built CPython wheels for x86-64 Unix platforms, as well as the code required to compile from source with Rust:
$ pip install pyskani
In the event you have to compile the package from source, all the required Rust libraries are vendored in the source distribution, and a Rust compiler will be setup automatically if there is none on the host machine.
🔖 Citation
If you found Pyskani useful, please cite our paper, as well as the original skani paper.
To cite Pyskani:
Martin Larralde, Georg Zeller, Laura M. Carroll. 2025. PyOrthoANI, PyFastANI, and Pyskani: a suite of Python libraries for computation of average nucleotide identity. NAR Genomics and Bioinformatics 7(3):lqaf095. doi:10.1093/nargab/lqaf095.
To cite skani:
Jim Shaw, Yun William Yu. 2023. Fast and robust metagenomic sequence comparison through sparse chaining with skani. Nature Methods 20(11):1661-1665. doi:10.1038/s41592-023-02018-3.
💡 Examples
📝 Creating a database
A database can be created either in memory or using a folder on the machine filesystem to store the sketches. Independently of the storage, a database can be used immediately for querying, or saved to a different location.
Here is how to create a database into memory, using Biopython to load the record:
database = pyskani.Database()
record = Bio.SeqIO.read("vendor/skani/test_files/e.coli-EC590.fasta", "fasta")
database.sketch("E. coli EC590", bytes(record.seq))
For draft genomes, simply pass more arguments to the sketch method, for
which you can use the splat operator:
database = pyskani.Database()
records = Bio.SeqIO.parse("vendor/skani/test_files/e.coli-o157.fasta", "fasta")
sequences = (bytes(record.seq) for record in records)
database.sketch("E. coli O157", *sequences)
🗒️ Loading a database
To load a database, either created from skani or pyskani, you can either
load all sketches into memory, for fast querying:
database = pyskani.Database.load("path/to/sketches")
Or load the files lazily to save memory, at the cost of slower querying:
database = pyskani.Database.open("path/to/sketches")
🔎 Querying a database
Once a database has been created or loaded, use the Database.query method
to compute ANI for some query genomes:
record = Bio.SeqIO.read("vendor/skani/test_files/e.coli-K12.fasta", "fasta")
hits = database.query("E. coli K12", bytes(record.seq))
🔎 See Also
Computing ANI for closed genomes? You may also be interested in
pyfastani, a Python package for computing ANI
using the FastANI method
developed by Chirag Jain et al.
💭 Feedback
⚠️ Issue Tracker
Found a bug ? Have an enhancement request ? Head over to the GitHub issue tracker if you need to report or ask something. If you are filing in on a bug, please include as much information as you can about the issue, and try to recreate the same bug in a simple, easily reproducible situation.
🏗️ Contributing
Contributions are more than welcome! See
CONTRIBUTING.md
for more details.
⚖️ License
This library is provided under the MIT License.
The skani code was written by Jim Shaw
and is distributed under the terms of the MIT License
as well. See vendor/skani/LICENSE for more information. Source distributions
of pyskani vendors additional sources under their own terms using
the cargo vendor
command.
This project is in no way not affiliated, sponsored, or otherwise endorsed
by the original skani authors.
It was developed by Martin Larralde during his
PhD project at the European Molecular Biology Laboratory
in the Zeller team.
📚 References
- [1] Jim Shaw and Yun William Yu. 'Fast and robust metagenomic sequence comparison through sparse chaining with skani' (2023). Nature Methods. doi:10.1038/s41592-023-02018-3. PMID:37735570.
Release files for pyskani 0.2.0
For a detailed explanation of source distributions (sdists) and built distributions (wheels), please see the package formats documentation.
Source distribution (sdist)
| File | Size | Uploaded | |
|---|---|---|---|
| pyskani-0.2.0.tar.gz | 2.9 MB | Details |
Built distributions (wheels)
Total release size: 81.7 MB
Release files / pyskani-0.2.0.tar.gz
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