Immunological peptide datasets and amino acid properties
An important aspect of computational immunology is modeling the properties of peptides (short strings of amino acids). Peptides can arise as substrings cut out of a larger protein, naturally occurring small proteins, or be synthesized for therapeutic purposes. To make useful clinical and research predictions (i.e. “which peptides should go in this vaccine?”) we need to partition the combinatorial space of peptides into classes such as T-cell epitopes or MHC ligands. One way to capture such distinctions is to collect large volumes of data about peptides and use that data to build statistical models of their immune properties. This library helps you build such models by providing simple Python/NumPy/Pandas interfaces to commonly used immunology and bioinformatics datasets.
When a dataset consists only of an unlabeled list of epitopes, then it only needs two functions: - load_wuzzle: Returns set of amino acid strings - load_wuzzle_ngrams: Array whose rows are amino acids transformed into n-gram vector space.
If the dataset contains additional data about the epitopes (such as HLA type u or source protein): - load_wuzzle: Returns data frame with epitope strings and additional properties - load_wuzzle_set: Set of epitope amino acid strings - load_wuzzle_ngrams: Array whose rows are amino acids transformed into n-gram vector space.
If the dataset is labeled (contains positive and negative assay results), then the following functions should be available: - load_wuzzle: Load all available data from the “wuzzle” dataset (filtered by options such as mhc_class). - load_wuzzle_values: Group the dataset by epitope string and associate each epitope with the positive and negative counts, along with percentage of positive results (in a column called “value”). - load_wuzzle_classes: Split the epitopes into positive and negative classes, return a set of strings for each. - load_wuzzle_ngrams: Transform the amino acid string representation (or some reduced alphabet) into vectors of n-gram frequencies, return a sklearn-compatible (samples, labels) pair of arrays.
Amino Acid Properties
The amino_acid module contains a variety of physical/chemical properties for both single amino residues and interactions between pairs of residues.
Single residue feature tables are parsed into StringTransformer objects, which can be treated as dictionaries or will vectorize a string when you call their method transform_string.
Examples of single residue features: - hydropathy - volume - polarity - pK_side_chain - prct_exposed_residues - hydrophilicity - accessible_surface_area - refractivity - local_flexibility - accessible_surface_area_folded - alpha_helix_score (Chou-Fasman) - beta_sheet_score (Chou-Fasman) - turn_score (Chou-Fasman)
Pairwise interaction tables are parsed into nested dictionaries, so that the interaction between amino acids x and y can be determined from d[x][y].
Pairwise interaction dictionaries: - strand_vs_coil (and its transpose coil_vs_strand) - helix_vs_strand (and its transpose strand_vs_helix) - helix_vs_coil (and its transpose coil_vs_helix) - blosum30 - blosum50 - blosum62
There is also a function to parse the coefficients of the PMBEC similarity matrix, though this currently lives in the separate pmbec module.