bioinformatics modules.
Project description
biomodules: 生物信息模块
基础模块(base)
MergeDir,给定一个输入文件夹和一个输出文件,把该文件夹下的所有文件合并到该文件中;CountLines,给定一个文件计算其行数;MergeZipFile,给定一个zip文件,将该zip文件下的所有文件合并为一个文件
序列相关(seq)
Fastq2Fasta,fastq转fasta;SubSeq,从fasta文件中提取部分序列;
预处理模块(preprocessing)
Fastqc,使用fastqc进行质量控制;Filtlong,使用Filtlong过滤reads;
序列比对(align)
Blastn,使用blastn进行序列比对;Minimap2,使用minimap2进行序列比对;Samtools,使用samtools处理比对结果;LowDepth,提取samtools depth中低深度位点;Muscle,muscle多序列比对;
变异检测(variant)
Bgzip,bgzip压缩;Bcftools,bcftools相关功能;
Oxford Nanopore(ont)
注意,使用该功能需要安装额外的包:
pip install biomodules[ont]
medaka,使用medaka call突变;
系统发育
IQTree,使用iqtree构建进化树MSAPhyloTree,使用多序列比对构建进化树
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