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bioinformatics modules.

Project description

biomodules: 生物信息模块

基础模块(base)

  1. MergeDir,给定一个输入文件夹和一个输出文件,把该文件夹下的所有文件合并到该文件中;
  2. CountLines,给定一个文件计算其行数;
  3. MergeZipFile,给定一个zip文件,将该zip文件下的所有文件合并为一个文件

序列相关(seq)

  1. Fastq2Fasta,fastq转fasta;
  2. SubSeq,从fasta文件中提取部分序列;

预处理模块(preprocessing)

  1. Fastqc,使用fastqc进行质量控制;
  2. Filtlong,使用Filtlong过滤reads;

序列比对(align)

  1. Blastn,使用blastn进行序列比对;
  2. Minimap2,使用minimap2进行序列比对;
  3. Samtools,使用samtools处理比对结果;
  4. LowDepth,提取samtools depth中低深度位点;
  5. Muscle,muscle多序列比对;

变异检测(variant)

  1. Bgzip,bgzip压缩;
  2. Bcftools,bcftools相关功能;

Oxford Nanopore(ont)

注意,使用该功能需要安装额外的包:

pip install biomodules[ont]
  1. medaka,使用medaka call突变;

系统发育

  1. IQTree,使用iqtree构建进化树
  2. MSAPhyloTree,使用多序列比对构建进化树

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