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https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat-square) https://badge.fury.io/py/sequana.svg https://github.com/sequana/sequana/actions/workflows/main.yml/badge.svg?branch=main https://coveralls.io/repos/github/sequana/sequana/badge.svg?branch=main Documentation Status JOSS (journal of open source software) DOI
Python version:

3.7, 3.8, 3.9

Documentation:

On readthedocs

Issues:

On github

How to cite:

Citations are important for us to carry on developments. For Sequana library (including the pipelines), please use

Cokelaer et al, (2017), ‘Sequana’: a Set of Snakemake NGS pipelines, Journal of Open Source Software, 2(16), 352, JOSS DOI doi:10.21105/joss.00352

For the genome coverage tool (sequana_coverage): Dimitri Desvillechabrol, Christiane Bouchier, Sean Kennedy, Thomas Cokelaer http://biorxiv.org/content/early/2016/12/08/092478

For Sequanix: Dimitri Desvillechabrol, Rachel Legendre, Claire Rioualen, Christiane Bouchier, Jacques van Helden, Sean Kennedy, Thomas Cokelaer. Sequanix: A Dynamic Graphical Interface for Snakemake Workflows Bioinformatics, bty034, https://doi.org/10.1093/bioinformatics/bty034 Also available on bioRxiv (DOI: https://doi.org/10.1101/162701)

Sequana includes a set of pipelines related to NGS (new generation sequencing) including quality control, variant calling, coverage, taxonomy, transcriptomics. We also ship Sequanix, a graphical user interface for Snakemake pipelines.

Pipelines and tools available in the Sequana project

name/github

description

Latest Pypi version

Test passing

sequana_pipetools

Create and Manage Sequana pipeline

https://badge.fury.io/py/sequana-pipetools.svg https://github.com/sequana/sequana_pipetools/actions/workflows/main.yml/badge.svg

sequana-wrappers

Set of wrappers to build pipelines

Not on pypi

https://github.com/sequana/sequana-wrappers/actions/workflows/main.yml/badge.svg

demultiplex

Demultiplex your raw data

https://badge.fury.io/py/sequana-demultiplex.svg https://github.com/sequana/demultiplex/actions/workflows/main.yml/badge.svg

fastqc

Get Sequencing Quality control

https://badge.fury.io/py/sequana-fastqc.svg https://github.com/sequana/fastqc/actions/workflows/main.yml/badge.svg

mapper

Map sequences on target genome

https://badge.fury.io/py/sequana-mapper.svg https://github.com/sequana/mapper/actions/workflows/main.yml/badge.svg

pacbio_qc

Pacbio quality control

https://badge.fury.io/py/sequana-pacbio-qc.svg https://github.com/sequana/pacbio_qc/actions/workflows/main.yml/badge.svg

ribofinder

Find ribosomal content

https://badge.fury.io/py/sequana-ribofinder.svg https://github.com/sequana/ribofinder/actions/workflows/main.yml/badge.svg

rnaseq

RNA-seq analysis

https://badge.fury.io/py/sequana-rnaseq.svg https://github.com/sequana/rnaseq/actions/workflows/main.yml/badge.svg

variant_calling

Variant Calling

https://badge.fury.io/py/sequana-variant-calling.svg https://github.com/sequana/variant_calling/actions/workflows/main.yml/badge.svg

multicov

Coverage (mapping)

https://badge.fury.io/py/sequana-multicov.svg https://github.com/sequana/multicov/actions/workflows/main.yml/badge.svg

laa

Long read Amplicon Analysis

https://badge.fury.io/py/sequana-laa.svg https://github.com/sequana/laa/actions/workflows/main.yml/badge.svg

revcomp

reverse complement of sequence data

https://badge.fury.io/py/sequana-revcomp.svg https://github.com/sequana/revcomp/actions/workflows/main.yml/badge.svg

downsampling

downsample sequencing data

https://badge.fury.io/py/sequana-downsampling.svg https://github.com/sequana/downsampling/actions/workflows/main.yml/badge.svg
Pipelines not yet released

name/github

description

Latest Pypi version

Test passing

trf

Find repeats

https://badge.fury.io/py/sequana-trf.svg https://github.com/sequana/trf/actions/workflows/main.yml/badge.svg

multitax

Taxonomy analysis

https://badge.fury.io/py/sequana-multitax.svg https://github.com/sequana/multitax/actions/workflows/main.yml/badge.svg

Please see the documentation for an up-to-date status and documentation.

Contributors

Maintaining Sequana would not have been possible without users and contributors. Each contribution has been an encouragement to pursue this project. Thanks to all:

https://contrib.rocks/image?repo=sequana/sequana

Changelog

Version

Description

0.14.3

  • new fisher metric in variant calling

  • ability to use several feature in rnaseq/rnadiff

  • pin several libaries due to regression during installs

0.14.2

  • Update ribodesigner

0.14.1

  • Kegg enrichment: add gene list ‘all’ and fix incomplete annotation case

  • New uniprot module for GO term enrichment and enrichment refactorisation (transparent for users)

0.14.0

  • pinned click>=8.1.0 due to API change (autocomplete)

  • moved tests around to decrease packaging from 16 to 4Mb

  • ribodesigner: new plots, clustering and notebook

0.13.X

  • Remove useless standalones or moved to main sequana command

  • Move sequana_lane_merging into a subcommand (sequana lane_merging)

  • General cleanup of documentation, test and links to pipelines

  • add new ribodesigner subcommand

0.12.7

  • Fix memory leak in len() of FastA class

0.12.6

0.12.5

  • refactorisation of VCF tools/modules to use vcfpy instead of pyVCF

0.12.4

  • complete change log before 0.12.4 on readthedocs.org

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